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Use of stephanotis extract in preparing antineoplastic agents

A technology of antineoplastic drugs and Baiyaozi, which is applied in the direction of antineoplastic drugs, drug combinations, and pharmaceutical formulas, and achieves the effect of low cost and simple method

Inactive Publication Date: 2008-11-19
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the application of Baiyaozi in the preparation of antitumor drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of Baiyaozi extract

[0020] Get Baiyaozi (Beijing Tongrentang Co., Ltd.) 200g, mix it with 95% ethanol (ethanol: water=95:5, volume ratio) with the weight and number ratio of 1: 3 (Baiyaozi: 95% ethanol), in Reflux extraction at 60°C for 3 hours, filter with suction, collect the first reflux extract 1 and filter residue 1; mix all the obtained filter residue 1 with 95% ethanol, the amount of 95% ethanol is the same as the first time, at 60°C Extract under reflux for 3 hours, collect the second reflux extract 2 and filter residue 2, and combine the extract 2 with the extract 1; mix all the filter residue 2 obtained with 95% ethanol, and the amount of 95% ethanol is the same as the first time , reflux extraction at 60°C for 3h, suction filtration, and collect the third reflux extract 3; combine the extract 1, extract 2 and extract 3, concentrate the obtained extract under reduced pressure and vacuum dry (remove ethanol and water) to constan...

Embodiment 2

[0021] Example 2, Application of Baiyaozi Extract in Inhibiting Tumor Cell Growth

[0022] The logarithmically grown tumor cells (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung cancer cell A-549) were digested with 0.25% trypsin, and then treated with 10% (volume percentage content) DMEM complete medium of fetal bovine serum suspends cells, makes cell suspension, and dilutes each tumor cell to 1.0×10 respectively 5 cells / mL, and then inoculated into 96-well culture plate, 200uL per well, each tumor cell was inoculated into 3 wells respectively, at 37°C, 5% CO 2 Cultivate in an incubator for 24 hours; then pour out the culture solution in each well.

[0023] The Baiyaozi extract prepared in Example 1 is dissolved in DMSO, then diluted with DMEM complete medium to obtain the Baiyaozi extract solution, the final concentration of Baiyaozi extract is 50ug / mL, and the concentration of DMSO is 10% (volume percentage).

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PUM

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Abstract

The invention discloses a new use of stephanotis extract, and the new use is the application of the stephanotis extract in preparation of antineoplastic medicaments. The extract is prepared according to the following method: the stephanotis extract is extracted from stephanotis with water and / or an organic solvent as an extractant. The stephanotis extract provided by the invention is very important for preparing antineoplastic medicaments, in particular anti-liver cancer, anti-lung cancer, and anti-colon cancer medicaments.

Description

technical field [0001] The invention relates to the application of Baiyaozi extract in the preparation of antitumor drugs. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage, but once symptoms appear and go to the doctor, it is already in the middle and late stages. Therefore, the treatment of liver cancer is very difficult, and the average patient's survival time after onset is only 6 months, which is called "the king of cancer". According to the survey results, the top three new cancers in men are lung cancer, colon cancer, and nasopharyngeal cancer, and breast cancer, colon cancer, and lung cancer in women, which fully demonstrates the harm of lung cancer and colon cancer in the new cancer group sex. [0003] ...

Claims

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Application Information

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IPC IPC(8): A61K36/59A61P35/00A61K125/00
Inventor 唐亚林谭莉戴玮刘岩向俊锋
Owner INST OF CHEM CHINESE ACAD OF SCI
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