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Method for evaluating DNA damage repair ability genetic risk

A genetic risk assessment and DNA damage technology, applied in the field of molecular biology, can solve problems such as limited popularization and application, high cost, and insufficient safety

Inactive Publication Date: 2008-11-12
上海中优医药高科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of CAT requires radioactive isotopes, which is not safe enough, and the cost is high, which limits its popularization and application.

Method used

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  • Method for evaluating DNA damage repair ability genetic risk
  • Method for evaluating DNA damage repair ability genetic risk
  • Method for evaluating DNA damage repair ability genetic risk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A method for genetic risk assessment of DNA damage repair ability is:

[0080] Step 1. Types of samples tested and sampling methods:

[0081] Use sampling swabs in the oral cavity of 26 subjects to scrape the left and right cheeks at least 45 times, so as to ensure that sufficient cell samples are collected;

[0082] Step 2. Extraction method of genomic DNA:

[0083] The genomic DNA of each oral epithelial cell sample was extracted by silica gel adsorption for 2 hours, and a total of 26 samples were extracted, and a clear white strip could be seen with the naked eye to judge that the obtained DNA could enter the next step of detection;

[0084] Step 3: Real-time quantitative PCR reaction

[0085] Put each sample of the subject into 5 reaction wells, and detect 5 sites at the same time, that is, poly ADP ribosyltransferase (PARP1) SEQ ID NO1: Val762Ala, human X-ray staggered complementary repair gene 1 (XRCC1) SEQ ID NO2: Arg194Trp, human X-ray staggered complementati...

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Abstract

The invention discloses a method for DNA damage repair capacity inheritance risk assessment which is characterized in that: by simultaneously detecting and analyzing carrying types of a PARP1 gene, an XRCC1 gene, an ERCC2 and an SNPs locus gene of an individual, the method provides all examinees with personalized health guidance of inheritance risk assessment and related disease risk aversion of DNA damage repair capacity. The method has the advantages that: an intervention measure can be taken before a gene damage happens, thereby avoiding the gene damage further to reduce the incidence of disease and the death rate of diseases such as tumor diseases related to the gene damage; the method analyzes a tumor pathogenic mechanism from a molecular mechanism, and can be used for the formulation of molecular cancer epidemiology investment and tumor prevention measures.

Description

technical field [0001] The invention relates to a method for genetic risk assessment of DNA damage repair ability, which is used to evaluate the strength of gene damage repair ability, and specifically prompts the subject to take appropriate responses to various external factors that may cause gene damage The method belongs to the technical field of molecular biology. Background technique [0002] In the process of genomic nucleic acid replication, there are complex mechanisms in cells to ensure that genetic information is replicated correctly, because the genetic conservation of DNA is the main factor to maintain the relative stability of species. However, under the influence of various factors in the internal and external environment of organisms, DNA damage, also known as DNA mutation, will also occur. Most of the damage is repaired by a set of very effective repair systems in the body, and some damage can cause irreversible damage to the body, such as cell aging, death ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 傅咏南王校毛丹丹
Owner 上海中优医药高科技股份有限公司
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