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Method for installing tube in rat spinal dural external cavity and subarachnoid cavity

A subarachnoid space and epidural technology, applied in medical science, veterinary instruments, etc., can solve the problems of complex operation, large animal damage, and short catheterization time, achieving a high success rate of catheterization and low animal injury. The effect of small sex and accurate positioning

Inactive Publication Date: 2008-11-12
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the development and research of natural active peptides and protein new drugs, it is necessary to insert medication into the epidural space and subarachnoid space of experimental animals, and to insert medication into the epidural space and subarachnoid space of experimental animals ( Especially long-term intubation drug) problem has not been effectively resolved
These methods are more complicated to operate, more harmful to animals, and the catheterization time is short and the success rate is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: The embodiment of the catheterization method of the thoracic vertebrae in the epidural space of rats

[0026] (1) Make the rat under anesthesia, and the operator touches the spinous process of the thoracic spine with fingers on the back of the anesthetized animal from the head to the tail. The most prominent spinous process in the thoracic region is the thoracic spine 8 , take this as the center, use hair scissors to cut the mouse hair within the range of about 5 × 2cm in diameter, and wipe and disinfect the skin area for 3 times with a complex iodine cotton swab; (2) under sterile conditions, use the chest 8 With the spinous process as the center, make a skin incision about 1.5 cm long parallel to the spine, and use the opener to open the skin and subcutaneous fat in sequence to clearly expose the back muscles and tendons of the rat; (3) Move the opener toward the lumbar spine of the rat , by identifying the tendon attachment point to determine the thorac...

Embodiment 2

[0028] Example 2: Example of catheterization in the foramen magnum in the epidural space of rats

[0029] Make the rats under anesthesia, make an incision about 1.5cm long along the dorsal midline under the occipital bone, expose the first cervical vertebra, expose the posterior atlanto-occipital membrane, carefully draw a small hole in the arachnoid, and insert a PE10 tube to the subarachnoid cavity, PE10 tube into the tube depth of about 7cm, to the lumbar enlargement of the spinal cord. The outer end is left about 5cm long, the tube is buried, and the incision is sutured. All the other operating steps are the same as in Example 1.

Embodiment 3

[0030] Embodiment 3: Embodiment of the method of inserting a catheter in the subarachnoid cavity of the rat

[0031] After exposing the oval hole between chest 8 and chest 9, use small ophthalmic scissors to insert into the spinal canal through the oval hole, carefully cut off the bony tissue around the hole, and enlarge the hole to fully expose Spinal cord: use a hook needle to break the dura mater, and a little clear cerebrospinal fluid can be seen flowing out; use ophthalmic forceps to clamp the special PE-10 tube first through a muscle tunnel, and insert it into the subarachnoid space of the rat along the dura mater hole , the insertion depth is about 1.8cm, reaching directly to the lumbar enlargement of the spinal cord; the rest of the operation steps are the same as in Example 1.

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Abstract

The invention relates to a method for placing a tube inside a rat spinal dura mater outer cavity and a subarachnoid cavity. During placing a tube, the method lifts a vertebral column by means of a hook needle so as to fully expose a vertebra gap between a chest 9 and a chest 10 without stripping the tissues beside the vertebral column such as muscles and fasciae; the hook needle is used to lift the vertebral column by means of a natural pore between the vertebral laminas between a chest 8 and a chest 9, thereby fully exposing a pore between vertebras without stripping and shearing off the muscles at both sides of the vertebral column; moreover, the tube can be exactly placed inside a rat spinal dura mater outer cavity for a longer time without adopting a tong to clamp the vertebras; in addition, during placing a tube inside a subarachnoid cavity, only a small amount of bony tissue around a natural pore between vertebras is bitten off by a tong. The method has the advantages of simple operation, less damage on an animal, accurate positioning and high success ratio during placing a tube; moreover, the method can be used in the research and development of new drugs of the same type as well as various animal experiments in preclinical pharmacodynamics, pharmacology and toxicology, etc.

Description

technical field [0001] The invention relates to a method for placing catheters in the epidural space and subarachnoid space of rats. Background technique [0002] Extracting active substances from natural toxins, especially active polypeptides and protein substances, and developing them into drugs is a research hotspot in the field of medicine and biology. However, in the development and research of natural active peptides and protein new drugs, it is necessary to insert medication into the epidural space and subarachnoid space of experimental animals, and to insert medication into the epidural space and subarachnoid space of experimental animals ( Especially long-term intubation medication) problem has not been effectively resolved. [0003] In 2002, the inventor referred to human epidural space and subarachnoid space clinical puncture method and x-ray angiography positioning, and adapted and improved according to different types and differences of animals, and established...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
Inventor 陈嘉勤梁宋平钟文涛张勇群毛海峰陈威华罗学锋
Owner HUNAN NORMAL UNIVERSITY
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