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Promoter for expressing phosphor deficiency speciality induction in root and use thereof

A promoter and phosphorus deficiency technology, applied in the field of promoters, can solve the problems of difficulty in meeting the needs of plant growth and development, low phosphorus, and low effective phosphorus concentration.

Inactive Publication Date: 2008-11-05
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The absolute phosphorus content in the soil is usually high (>1000μM), but phosphorus is easily fixed in organic and inorganic forms in the soil, so the concentration of available phosphorus is very low, about 2-10μM, and the phosphorus diffused to the root surface is even lower. Difficult to meet the needs of plant growth and development

Method used

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  • Promoter for expressing phosphor deficiency speciality induction in root and use thereof
  • Promoter for expressing phosphor deficiency speciality induction in root and use thereof
  • Promoter for expressing phosphor deficiency speciality induction in root and use thereof

Examples

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Effect test

Embodiment 1

[0022] Embodiment 1, the isolation of wheat phosphate radical transporter gene TaPHT1.2 promoter sequence

[0023] The genomic DNA of wheat variety Xiaoyan 54 was extracted, and the wheat genomic DNA was completely digested with four blunt-ended Dra I, EcoR V, Pvu II and Stu I using the Universal Kit of Clontech Company, and then digested with T 4 DNA Ligase adds adapters to both ends of the enzyme-digested fragments, and designs two 5' primers based on the sequences on the adapters, and designs two nested-PCR primers at the 3' end based on the known TaPHT1.2 gene sequence:

[0024] The linker sequence is as follows:

[0025] 5′-GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGGT-3′

[0026] 3′-H2N-CCCGACCA-PO4-5′

[0027] The 5' end primer sequence is as follows:

[0028] Adapter primer 1: 5′-GTA ATA CGA CTC ACT ATA GGG C-3′;

[0029] Adapter primer 2: 5'-ACT ATA GGG CAC GCG TGG T-3'.

[0030] The 3' end primer sequence is as follows: (gene-specific primer)

[0031] Gene-s...

Embodiment 2

[0035] Example 2, Functional Analysis of Wheat Phosphate Transporter Gene TaPHT1.2 Promoter

[0036] 1) Analysis of promoter expression characteristics

[0037] Cut out the TaPHT1.2 promoter fragment from PUC18 T-TaPHT1.2 with endonucleases Hind III and BamH I, and use these two enzymes to cut the 35S promoter part of the download vector pBI121, insert the TaPHT1.2 promoter Between the Hind III and BamH I sites of pBI121, construct the pBI121-TaPHT1.2-GUS recombinant expression vector ( figure 1 ), the constructed recombinant expression vector of pBI121-TaPHT1.2-GUS was transformed into Agrobacterium GV3101.

[0038]The EGFP (Cat. #4999-100, Clontech, Palo Alto, CA) coding region was cloned into the pJIT163 (Guerineau et al., Plant Molecular Biology, 1992, 18: 815-818) vector (Research on Genetics and Developmental Biology, Chinese Academy of Sciences Downstream of the 35S promoter, the pJIT163-GFP vector was obtained. Cut the TaPHT1.2 promoter fragment from PUC18 T-TaPHT1....

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Abstract

The invention discloses a phosphorus deficiency specific inducement root-expression promoter and applications thereof. The promoter can be a DNA molecule as the following 1), 2) or 3): (1) the ribonucleotide sequence thereof is a DNA molecule in the 1st-serial in a sequence list; (2) a DNA molecule which can be hybridized with the DNA molecule of the 1st-serial in the sequence list under a strict condition and can drive targeted genes to be expressed specially in root in case of phosphorus deficiency; (3) a DNA molecule which has the homeology with the DNA by more than 90 percent and can drive targeted genes to be expressed specially in root in case of phosphorus deficiency. The promoter of the invention can be applied to the construction of carriers with economic value and the constructed carriers can be used for breeding transgenic plants with economic value.

Description

technical field [0001] The present invention relates to a promoter expressing in roots specifically induced by phosphorus deficiency and its application. Background technique [0002] Promoter is the most important factor among gene expression regulators, which basically determines whether, when and where a gene is expressed. According to the mode of action and function, promoters can be roughly divided into three categories: constitutive promoters, specific promoters and inducible promoters. The characteristic of an inducible promoter is that the gene controlled by this type of promoter is not expressed or has very low expression in the absence of an inducing factor, but once induced by an inducing factor, the expression of the gene increases rapidly and significantly. For example: hormone-inducible promoters, chemical-inducible promoters, light-inducible promoters, etc. The characteristics of constitutive promoters are that the expression of structural genes controlled b...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H1/00C12N15/113
Inventor 童依平缪军李振声李滨
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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