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Mangrove rhizosphere growth promoting azotobacter (DZY-HS14) and use thereof

A mangrove plant, rhizosphere growth promotion technology, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve problems such as low survival rate, and achieve the effect of improving productivity

Inactive Publication Date: 2008-11-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the survival rate of introduced strains in our country's mangroves is low, and there are also various hidden dangers caused by foreign species invasion

Method used

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  • Mangrove rhizosphere growth promoting azotobacter (DZY-HS14) and use thereof
  • Mangrove rhizosphere growth promoting azotobacter (DZY-HS14) and use thereof
  • Mangrove rhizosphere growth promoting azotobacter (DZY-HS14) and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Materials and methods

[0029] 1. Materials

[0030] 1.1 Soil sample collection

[0031] The samples were collected from the rhizosphere sediments of mangrove plants in the mangrove area along the Hongsha River in Sanya City, Hainan Province. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory for low temperature storage.

[0032] 1.2 Medium

[0033] Ashby Broth: Mannitol 10.0g, KH 2 PO 4 ·H 2 O 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, CaSO 4 0.1g, CaCO 3 5.0g, 1000mL deionized water, pH 7.0.

[0034] Ashby solid medium: Ashby liquid medium + 2.2% agar.

[0035] to improve Nitrogen-free liquid medium: sucrose 10g, malic acid 5.0g, K 2 HPO 4 ·H 2 O 0.1g, KH 2 PO 4 ·H 2 O0.4g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, NaCl 2 MOO 4 ·H 2 O 0.002g, FeCl 3 0.01g, 1000ml deionized water, pH 7.0.

[0036] LB medium: yeast extract 5g, tryptone 10g, NaCl 10g, agar 1-2%, deionized water 1000ml...

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Abstract

The invention provides a mangrove plant rhizosphere growth-promoting nitrogen fixing bacterium (DZY-HS14) and an application thereof. The bacterium is the bacillus sp. DZY-HS14 screened from the rhizosphere settlings of the tropical mangrove plants and contains 16SrDNA serial and nifH and is stored in the CCTCC; the collection number is CCTCCNO: M207043. The strain has high biological nitrogen fixing activity; the microbial inoculum with the bacterium is used to breed mangrove plants such as rhizophora stylosa and bruguiera, which can evidently promote the growth of plants, thereby being capable of preparing the biological nitrogen fixing bacterium fertilizer promoting the growth of mangrove plants.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and in particular relates to a mangrove rhizosphere nitrogen-fixing bacterium having a good growth-promoting effect on mangrove plants and an application thereof for preparing biological nitrogen-fixing bacterial fertilizer. Background technique: [0002] Mangroves are woody plant communities naturally distributed in the intertidal zone of tropical and subtropical coasts, covering about 60-75% of tropical and subtropical coasts, playing an important role in maintaining the ecological balance of bays and estuaries, and are an important part of marine productivity (Holguin et al., 2001), considered to be a highly productive ecosystem. The ecosystem has high levels of organic matter (100 TgC per year in global litter), providing organic matter to adjacent coastal waters and associated biome habitats (Holguin et al., 2001; Lugomela and Bergman, 2002). However, mangrove ecosystems are poor in in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07
Inventor 董俊德杨志浩张燕英凌娟王友绍张偲吴梅林
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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