Bone-melon extract injection and preparation thereof
A technology of extract and bone melon, which is applied in the field of polypeptide pharmaceutical compositions, can solve the problems of low active ingredients and low extraction rate of bone melon extract injection, achieve low polymer content, high active ingredient content, and high yield of finished products. high rate effect
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Embodiment 1
[0052] The preparation of embodiment 1 pig bone extract
[0053] Stripping: Take fresh or frozen pig limb bones, remove the flesh and fascia, cut off, remove the bone marrow, and obtain 15kg of clean bones.
[0054] Extraction: Take the clean bone, add 4 times the weight of purified water, extract under hot pressure at 121°C and 0.12Mpa for 2 hours, filter to obtain the filtrate, and after the filtrate stands still at 5°C for 12 hours, remove the upper layer of oil from the extract, Use 8% sodium hydroxide solution to adjust the pH to 8.5, heat to about 45°C, add trypsin according to 0.2% of the bone weight, after constant temperature continuous hydrolysis for 2 hours, adjust the pH to 3.5 with 12% hydrochloric acid, boil, and keep warm for 30 minutes Filter and adjust the pH of the filtrate to 6.5 with 8% sodium hydroxide solution. Add activated carbon according to 0.1% of the volume of the filtrate, heat to boiling, keep warm for 15 minutes and then filter. The filtrate is ...
Embodiment 2
[0057] The preparation of embodiment 2 deer bone extract
[0058] Stripping: Take fresh or frozen sika deer limb bones, remove flesh and fascia, cut off, remove bone marrow, and obtain 15kg of clean bones.
[0059] Extraction: Take the clean bone, add 3 times the weight of purified water, extract under hot pressure at 121°C and 0.12Mpa for 2 hours, filter to obtain the filtrate, and then remove the upper layer of oil from the extract after standing still at 4°C for 12 hours. Use 8% sodium hydroxide solution to adjust the pH to 9.0, heat to about 45°C, add trypsin according to 0.2% of the bone weight, continue hydrolysis at constant temperature for 2 hours, adjust the pH to 4.0 with 12% hydrochloric acid, boil, and keep warm for 30 minutes Filter and adjust the pH of the filtrate to 7.0 with 8% sodium hydroxide solution. Add activated carbon according to 0.1% of the volume of the filtrate, heat to boiling, keep warm for 15 minutes and then filter. The filtrate is frozen at -20...
Embodiment 3
[0062] The preparation of embodiment 3 melon seed extract
[0063] Washing: Take the mature and dried melon seeds, rinse them with purified water for 4 times, dry the water, pick out the sundries, and get 15kg of clean melon seeds.
[0064] Extraction: Take clean melon seeds, add 2 times the weight of purified water, extract at 121°C, 0.12MPA hot press for 2 hours, filter to obtain the filtrate, then add 2 times the weight of purified water, repeat the last extraction, and repeat the extraction three times. The three filtrates were combined, cooled to 30°C, and the pH of the above filtrate was adjusted to 3.5 with 12% hydrochloric acid, and stood still for 12 hours.
[0065] Remove miscellaneous proteins, boil the above hydrolyzate, keep it warm for 30 minutes, filter it, adjust the pH of the above filtrate to 8.5 with 8% sodium hydroxide solution, freeze it at -20°C for 48 hours, then melt it at room temperature, boil it, and keep it warm for 30 minutes Filter and adjust the...
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Abstract
Description
Claims
Application Information
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