Method for preparing fatty acid by using lipase hydrolyzation of oil and fat

A technology for hydrolyzing oil and lipase, applied in the biological field, can solve the problems of increasing the hydrolysis rate of emulsifiers and speeding up the reaction process, and achieving the effects of less enzyme consumption, lower production costs, and increased collision opportunities

Active Publication Date: 2008-10-29
领先生物农业股份有限公司
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AI Technical Summary

Problems solved by technology

Only a few research articles have shown that the use of free or immobilized lipase (esterase) can be used for oil hydrolysis (Gu Wenling. Research progress in lipase oil hydrolysis technology. Western Grain and Oil, 1998, 23 (6): 31-35 Cao Guomin. Silk-immobilized lipase catalyzes the hydrolysis of olive oil. Journal of Petrochemical Universities, 1997, 10 (6): 35-37; etc.), the hydrolysis conditions studied are also limited to enzyme dosage, oil-water ratio, pH, stirring speed, etc., and there is no report about the use of emulsifiers to increase the hydrolysis rate and accelerate the reaction process

Method used

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  • Method for preparing fatty acid by using lipase hydrolyzation of oil and fat

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Add 2 grams of commercially available Koufu brand soybean oil into a 50 ml Erlenmeyer flask with a ground port stopper, then add 1.2 ml of enzyme solution prepared with pH6-pH7 phosphate buffer solution (the weight ratio of oil to water is 1: 0.6, and the enzyme powder is Lipase powder produced by Wuxi Enzyme Preparation Factory, enzyme activity 80U / ml), the pH of the aqueous phase was adjusted to 6.0 with 2N NaOH solution, and the shaking reaction was carried out at 40°C and 130 rpm for 36h. The acid value of the obtained fat hydrolyzate was determined by acidity analysis method to be 125 mgKOH / g oil, and the hydrolysis rate was calculated to be 62.5% according to the above method.

[0059] The obtained hydrolyzate was acidified with 2N sulfuric acid to make the pH value of the water phase reach 4.5, then heated to 68°C, allowed to stand for stratification, and the water phase (sweet water containing glycerin) was released to obtain crude fatty acid containing a small a...

Embodiment 2

[0063] Add 10 grams of palm oil sold under the trade name palm oil by Qinhuangdao Jinhai Cereals and Oils Industry Co., Ltd. in a 250 ml conical flask with a stopper, then add 0.1 g NOVOZYM435 (enzyme activity 10000 PLU / g, PLU / g refers to every 1g enzyme The propyl laurate unit that generates), adjust the weight ratio of oil and water with the phosphate buffer solution of pH6-7 to be 1: 0.2, add sodium stearate 1.4g, the pH of aqueous phase is adjusted to 5.7 with 2N NaOH solution, in 45°C, 130 rpm shaking reaction for 30h. The acid value of the hydrolyzed solution obtained was determined by acidity analysis method to be 162 mgKOH / g oil, and the hydrolysis rate was calculated to be 81% according to the above method.

[0064] The obtained hydrolyzate was acidified with 3N sulfuric acid to make the pH value of the water phase reach 4.5, then heated to 70°C, allowed to stand for stratification, and released the water phase (sweet water containing glycerin) to obtain crude fatty a...

Embodiment 3

[0067] Add 1000 grams of soybean acidified oil sold by Qinhuangdao Jinhai Cereals and Oils Industry Co., Ltd. into a 2000ml four-port reactor, add 600ml of enzyme solution prepared with pH6-pH7 phosphate buffer solution (the weight ratio of oil to water is 1:0.6, and the enzyme powder is Wuxi Lipase powder produced by the enzyme preparation factory, enzyme activity 80U / ml), sodium stearate 140g, use 2N NaOH solution to control the pH of the aqueous phase to 6.5-7.0, and react at 40°C and 100 rpm for 36h. The acid value of the obtained fat hydrolyzate was determined by acidity analysis method to be 143 mgKOH / g oil, and the hydrolysis rate was calculated according to the above method to be 71.5%.

[0068] The obtained hydrolyzate was acidified with 3N sulfuric acid to make the pH value of the water phase reach 4.5, then heated to 65°C, allowed to stand for stratification, and the water phase (sweet water containing glycerin) was released to obtain crude fatty acid containing a sm...

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Abstract

The invention discloses a method for producing fatty acid by hydrolyzing animal and vegetable oils or waste animal and vegetable oils using lipase. The method can achieve a hydrolysis rate above 90% by carrying out hydrolysis reaction using lipase in the presence of emulsifying agent. The enzymatic method of the invention is carried out under a mild condition to overcome the disadvantages of prior alkaline saponification, high-temperature high-pressure continuous cracking and medium-pressure cracking reaction, and has the advantages of less energy consumption, low equipment cost, no contaminant discharge, and high applicability for raw materials. Accordingly, the enzymatic method of the invention has wide application prospect.

Description

【Technical field】 [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for producing fatty acids using lipase. 【Background technique】 [0002] Fatty acid is an important chemical, daily chemical, light industry, pharmaceutical, and food raw material, especially in daily chemical, surfactant synthesis, and food. In the past, fatty acids were mainly produced from animal and vegetable oils by hydrolysis through physical and chemical methods. The reaction required heating, pressurization, or even high temperature and high pressure (for example, the continuous cracking method required 250°C, 5.5MPa), or under strong alkaline conditions ( Alkaline Saponification). Physicochemical lipolysis not only consumes a lot of energy, but also has high requirements for equipment due to severe reaction conditions. In addition, severe reaction conditions will increase side reactions and by-products, which will affect...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P7/20
Inventor 徐志文孟永宏周志奇王东李月娟
Owner 领先生物农业股份有限公司
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