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Preparation and application of compound for inhabiting aspergillus fumigatus activity

A compound, the technology of Aspergillus fumigatus, applied in the field of medicine, can solve the problem of no compound inhibiting the activity of Aspergillus fumigatus and other problems, and achieve the effect of inhibiting the activity of Aspergillus fumigatus

Inactive Publication Date: 2008-10-08
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In the literature Fungal metabolites, asterric acid derivatives inhibit vascular endothelial growth factor (VEGF)-induced tube formation of HUVECs Derivatives). J.Antibiot. (Journal of Antibiotics) 2002, 55, 552, the author reported that the compound methyl Estrel has inhibitory activity on VEGF, and there is no report on the activity of the compound to inhibit Aspergillis fumigatus (Aspergillis fumigatus)

Method used

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  • Preparation and application of compound for inhabiting aspergillus fumigatus activity
  • Preparation and application of compound for inhabiting aspergillus fumigatus activity
  • Preparation and application of compound for inhabiting aspergillus fumigatus activity

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: Preparation of Compound Methyl Esterui

[0028] a. Solid state fermentation of strain Geomyces sp.

[0029] Activation of strain Geomyces sp. PDA medium: 200g of potatoes, 20g of glucose, 15g of agar, 1000mL of water, sterilized by high-pressure steam at 121°C for 30 minutes, made into a test tube slant, picked mycelium and inoculated it on the test tube slant, and cultured at 25°C for 7 days;

[0030] Solid state fermentation of strain Geomyces sp. Preparation of rice culture medium (add 100g rice and 100mL water into a 500mL Erlenmeyer flask, soak overnight, sterilize with high pressure steam at 121°C for 30min, cool for later use); 6 Individuals / mL) 5 mL was inoculated on rice culture medium, and cultured in a sterile culture room at 25°C for 40 days.

[0031] b. Extraction of Methyl Estrela

[0032] After the fermentation was completed, ethyl acetate was added into the Erlenmeyer flask for extraction three times, and the organic solvent was distill...

Embodiment 2

[0044] Embodiment 2: The inhibition of the activity of Aspergillis fumigatus (Aspergillis fumigatus) detected by Alamar Blue fluorescence detection method

[0045] 1. Test reagents:

[0046] Use dimethyl sulfoxide (DMSO) as a solvent to prepare 4, 2, 1, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01, 0.005 and 0.002mg / mL of the tested solution;

[0047] The positive control substance fluconazole (purchased from Sigma Company) was prepared into a 4 mg / mL solution with DMSO;

[0048] 2. Cultivation of Aspergillis fumigatus

[0049] Aspergillus fumigatus (Aspergillis fumigatus 48238E, which was provided by the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences) was inoculated on PDB medium (starch 40 g / L, glucose 20 g / L), cultured on a shaker at 28 ° C for 2 days and then Count the number of spores under a microscope and adjust the number of spores to 1×10 5 individual / mL.

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Abstract

The invention relates to a methyl asterrate preparation method and the application thereof. The solid fermentation is performed to Fungi Geomyces sp, chemical compound methyl asterrate can be obtained by performing purification to fermentation products, and the chemical compound methyl asterrate prepared by the invention is confirmed methyl asterrate by applying technologies such as mass spectrum, nuclear magnetic resonance spectrum and infrared spectrum and documents. The invention also provides usages of the chemical compound in preparing drugs which can restraint the activity of aspergillus fumigatus.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method of a compound methyl asterrate and an application of the compound methyl asterrate in the preparation of a drug for inhibiting the activity of Aspergillus fumigatus. Background technique [0002] Aspergillus fumigatus is an opportunistic pathogenic fungus that seriously endangers industrial and agricultural production and human health. In a humid environment, Aspergillus fumigatus can infect corn, soybean meal and other grains, resulting in a decrease in grain yield and quality; Aspergillus fumigatus can also infect animals, and the toxin produced by Aspergillus fumigatus can cause equine leukomalacia, resulting in poultry weight loss, Diarrhea, low feed conversion efficiency and liver necrosis lead to pancreatic necrosis, liver damage and pulmonary edema in pigs. It can be seen that Aspergillus fumigatus can bring great harm to human industrial and agricul...

Claims

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Application Information

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IPC IPC(8): C12P7/62A61K31/216A61P31/10C12R1/645
Inventor 车永胜刘杏忠李彦孙炳达姜立花刘述春
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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