Alkaline soluble lentinan extraction, separation, purification and molecular weight determination

A technology of lentinan and molecular weight, which is applied in the field of new purification process and molecular weight determination, separation, and extraction of alkali-soluble lentinan, can solve the problems such as inability to accurately judge whether the raw material of lentinan is qualified or not, large error in molecular weight measurement, etc.

Inactive Publication Date: 2008-09-10
金文准 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different types of gel columns used, there is a large error in the determination of molecular weight, and it is impossible to accurately determine whether the raw material of lentinan is qualified

Method used

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  • Alkaline soluble lentinan extraction, separation, purification and molecular weight determination
  • Alkaline soluble lentinan extraction, separation, purification and molecular weight determination
  • Alkaline soluble lentinan extraction, separation, purification and molecular weight determination

Examples

Experimental program
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Effect test

Embodiment 1

[0011] Take 8 kg of qualified dry shiitake mushroom raw materials, crush them, and place them in a jacketed reaction pot. Add 80 kg of 0.5 mol / L NaoH alkali solution and heat to 40°C, keep stirring for 4 hours, filter, and extract the residue twice by the above method. Combine all the solutions, concentrate to one-tenth of the original volume, then add 0.3mol / L NaOH 2 times the lye of the concentrated solution to concentrate and wash twice, then use the same volume of deionized water to concentrate and wash twice, and the concentrated solution after washing The solution was detected by ninhydrin and the reaction did not show blue-purple. Use 50-70% ethanol to fractionate the washed concentrated solution under rapid stirring, continue to stir for 15 minutes and then stand still for 4 hours, the precipitation occurs, and the supernatant is poured out, and the precipitate is then washed with 80-90% ethanol After dissolving and stirring for 15 minutes, stand still for 4 hours, a ...

Embodiment 2

[0014] Take 10 kg of qualified dried shiitake mushroom raw materials, crush them, and place them in a jacketed reaction pot. Add 100 kg of 0.5mol / LnaoH alkali solution and heat to 40°C, keep stirring for 4 hours, filter, and extract the residue twice by the above method. Combine all the solutions, concentrate to one-tenth of the original volume, then add 0.3mol / L NaOH 2 times the lye of the concentrated solution to concentrate and wash twice, then use the same volume of deionized water to concentrate and wash twice, and the concentrated solution after washing The solution was detected by ninhydrin and the reaction did not show blue-purple. Use 50-70% ethanol to fractionate the washed concentrated solution under rapid stirring, continue to stir for 15 minutes and then stand still for 4 hours, the precipitation occurs, and the supernatant is poured out, and the precipitate is then washed with 80-90% ethanol After dissolving and stirring for 15 minutes, stand still for 4 hours, ...

Embodiment 3

[0017] Take 13 kilograms of qualified dried shiitake mushroom raw materials, crush them, and place them in a jacketed reaction pot. Add 130 kg of 0.5 mol / L naoH alkali solution and heat to 40°C, keep stirring for 4 hours, filter, and extract the residue twice by the above method. Combine all the solutions, concentrate to one-tenth of the original volume, then add 0.3mol / L NaOH 2 times the lye of the concentrated solution to concentrate and wash twice, then use the same volume of deionized water to concentrate and wash twice, and the concentrated solution after washing The solution was detected by ninhydrin and the reaction did not show blue-purple. Use 50-70% ethanol to fractionate the washed concentrated solution under rapid stirring, continue to stir for 15 minutes and then stand still for 4 hours, the precipitation occurs, and the supernatant is poured out, and the precipitate is then washed with 80-90% ethanol After dissolving and stirring for 15 minutes, it was left to s...

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Abstract

The invention discloses a new technology of extracting, separating and purifying 0.1-1 million Da alkali solubility lentinan and a method of molecular weight determination, by taking a mushroom fruit body as raw material, a crude product is obtained by grinding, potash leaching, filtering, concentrating, washing, grading and drying under low temperature, and then the crude product is dissolved and centrifuged, purified by a DEAE cellulose column and absorbed and discolored by ion exchange resin, and filtered to pass through membrane packets of different molecular weights for ultrafiltrating and concentrating. The molecular weight is measured by adopting a GPC laser light scattering gel chromatograph, and the obtained material is respectively put into dialysis bags for dialysis according to the measured molecular weight range, after freeze drying, the alkali solubility lentinan of various components with a molecular weight of 0.1-1 million Da and the content of more than 98 percent is obtained. The method has the advantages of advanced technology, stable quality, high purity, clear goal and easy industrialized production.

Description

technical field [0001] The invention is an improvement to the existing lentinan extraction process, and discloses a new extraction, separation and purification process of alkali-soluble lentinan and a new method for molecular weight determination. Extraction, separation, purification, preparation and detection methods involving natural biological response regulators are carried out using the fruiting bodies of Lentinus edodes as raw materials. Background technique [0002] Shiitake mushrooms are rich in protein, heteropolysaccharides, and lentinan. They are traditional Chinese medicinal materials in my country and have the effect of nourishing the liver and nourishing qi. Lentinan (English name Lentinan), whose primary structure is β-(1-3) glucose as the main chain, β-(1-6) glucose as the side chain of glucan, is an immune-regulating anti-tumor auxiliary drug. In 1965, after Chihara Goro of Japan discovered that it had anti-tumor activity, scientists from various countries...

Claims

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Application Information

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IPC IPC(8): G01N1/34G01N30/00
Inventor 金文准王务鼎王卫健周晓海胡荣大
Owner 金文准
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