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Method for producing phosphobacteria bio-fertilizer by using urban sludge

A technology of municipal sludge and biological fertilizer, applied in the direction of microorganism-based methods, sewage/sludge fertilizer, biochemical equipment and methods, etc., can solve the problems of low spore formation rate, high price, and high cost, and reduce production costs , promote popularization and application, and reduce disposal costs

Inactive Publication Date: 2008-09-03
SHANGHAI NUOTONG INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An authoritative department estimates that in the next five years, the annual demand for microbial fertilizers will reach 30% of chemical fertilizer consumption, and the market capacity will reach 42 million tons. However, the current annual production of microbial fertilizers in my country is less than 1 million tons, which is far from being able to meet market needs
[0007] The main production process of phosphorus bacterial bio-fertilizer is liquid submerged fermentation, and the main production raw materials are industrial and agricultural products such as peptone, corn flour, soybean powder, starch, yeast extract, etc. These raw materials have two defects: one is high cost; the other is phosphorus The fermentation time of bacteria in the fermenter is longer (generally 40-72 hours), and the sporulation rate is lower (generally 50-70%)
However, the medium is a synthetic medium composed of industrial and agricultural raw materials, and the price is still high
According to literature and patent searches, no report on the use of urban sludge as raw material to ferment and produce phosphorus bacterial bio-fertilizer has been found in the prior art

Method used

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  • Method for producing phosphobacteria bio-fertilizer by using urban sludge
  • Method for producing phosphobacteria bio-fertilizer by using urban sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Municipal sludge samples

[0037] Take the thickened sludge from a sewage treatment plant in Shanghai. The basic properties of the sludge are shown in Table 2.

[0038] Table 2 Basic properties of the tested sludge

[0039] pH

Solid content

(%)

total carbon

(%)

total nitrogen

(%)

Total Phosphorus

(%)

Cadmium

mg / kg

HG

mg / kg

lead

mg / kg

chrome

mg / kg

arsenic

mg / kg

7.39

3.30

30.5

2.32

1.17

1.2

0.7

63

115

12

[0040] (2) Sludge conditioning

[0041]Use tap water to adjust the solid content of the sludge to 3.0%, add 2.0g / L of antifoaming agent containing silicon-containing foam enemy XPJ960, adjust the pH to 6.9 with phosphoric acid, and test the pressure of 15 pounds and the temperature of 121°C in the seed tank and fermentation tank respectively. Sterilize for 60 minutes, cool to 35°C for later use...

Embodiment 2

[0058] (1) Municipal sludge samples

[0059] The properties of municipal sludge samples are the same as in Table 1.

[0060] (2) Sludge conditioning

[0061] Use tap water to adjust the solid content of the sludge to 2.0%, add 1.0g / L of antifoaming agent polyether antifoaming agent GPE, adjust the pH to 6.8 with phosphoric acid, and pass 15 pounds of pressure and 121°C in the seed tank and fermentation tank respectively. Sterilize for 60 minutes, cool to 35°C for later use, and the pH after sterilization is about 7.0.

[0062] (3) Shake flask culture

[0063] The strain was changed from Bacillus megaterium ACCC10010 to ACCC10099 strain, and the rest were the same as in Example 1.

[0064] (4) Seed tank fermentation

[0065] Inoculate 3.0% of the above-mentioned shake flask bacterial liquid, and ferment and cultivate. The culture conditions are: tank temperature 35° C., tank pressure 0.03 MPa, ventilation rate 1:1.0 (V / V / min), stirring speed 240 rpm, and fermentation time ...

Embodiment 3

[0074] (1) Municipal sludge samples

[0075] Take the thickened sludge from a sewage treatment plant in Guangzhou, Guangdong Province. The basic properties of the sludge are shown in Table 6.

[0076] Table 6 Basic properties of tested sludge

[0077] pH

[0078] (2) Sludge conditioning

[0079] Use tap water to adjust the solid content of municipal sludge to 4.0%, add 3g / L of antifoaming agent containing silicon foam enemy XPJ960, adjust the pH to 6.8 with sodium hydroxide, and pass 15 pounds of pressure and 121 in the seed tank and fermentation tank respectively. Sterilize at ℃ for 60 minutes, cool to 32℃ for later use, and the pH after sterilization is about 7.2.

[0080] (3) Shake flask culture

[0081] Pick a loop from the slant of Bacillus megaterium ACCC10099 and inoculate it into a triangular flask containing beef extract peptone liquid medium for shaking flask culture. The culture conditions are: temperature 32°C, rotation speed 200 rpm, 500 mL Erlenmeye...

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Abstract

The invention provides a low-cost, environment-friendly method for producing phosphorus bacteria biological fertilizer. The phosphorus bacteria biological fertilizer uses Bacillus megaterium as a production bacteria and sewage sludge as a production matrix and is produced through fermentation culture in seed tanks and fermentation tanks. The fermentation period of the whole growth process is shortened to 36-40 hours and bacillus formation rate is increased by 20%-40% compared with common methods and the live bacteria number in the fermentation liquid can reach 0.8-1 billion / ml and can reach 0.15-0.2 billion / g when the solution is absorbed with dry sterile peat. Moreover, the contents of cadmium, mercury, lead, chromium, arsenic and other heavy metals in the fermentation phosphorus bacteria fertilizer are much lower than the contents before fermentation. The pepper field experimental results show that the phosphorus bacteria biological fertilizer can increase the fertilizer utilization and output and benefit, and has positive effect to the agronomic traits such as the height of crops, width, weight, uniformity, the number of branches and the first harvest time, and meanwhile the comprehensive disease resistance is further enhanced.

Description

technical field [0001] The present invention relates to a kind of production method of biological fertilizer, in particular, the present invention relates to a kind of method of utilizing municipal sludge to produce phosphorus bacteria biological fertilizer. Background technique [0002] Municipal sludge is the sediment inevitably produced in the process of sewage treatment. At present, the annual output of urban sludge in my country is about 7 million tons of dry weight. According to the target of urban sewage treatment in my country, it is estimated that the amount of sludge generated by 2010 will exceed 8 million tons of dry weight. How to properly dispose of these increasingly large and highly concentrated organic solid wastes is one of the most troublesome problems for urban sewage treatment plants. [0003] Internationally, the current sludge disposal methods mainly include: landfill, land use, incineration, etc., but they all have their own insurmountable defects, an...

Claims

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Application Information

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IPC IPC(8): C05F7/00C05F11/08C05F11/02C12N1/20C12R1/11
CPCY02A40/20
Inventor 周顺桂李芳柏闫龙翔贾小红
Owner SHANGHAI NUOTONG INFORMATION TECH CO LTD
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