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Method for screening colorectal cancer by supermethylation of SFRP2 gene in dejecta

A technology of hypermethylation and colorectal cancer, applied in the medical field, can solve the problems of physical injury, low specificity and high cost, and achieve the effect of reducing the cost of testing, alleviating the pain of patients, and improving the efficiency of testing.

Inactive Publication Date: 2008-08-27
王道荣 +1
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AI Technical Summary

Problems solved by technology

These methods are either traumatic to the body, cause damage to the body, or are not specific, or the cost is too high. Therefore, to explore a safe, reliable, specific, and effective early screening method for colorectal cancer, To enable patients to detect, diagnose and treat colorectal cancer early has become a problem to be solved in the medical field

Method used

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Embodiment Construction

[0017] The patients collected stool samples by themselves, and the stool DNA was isolated from the stool samples (250 mg) with QIA&DNA Stool Mini Kit (Qiagen), and stored at -80°C for later use. 500 ng-2 μg of genomic DNA was denatured with NaOH (freshly prepared, final concentration 0.2 mol / L) in a volume of 50 μL at 37° C. for 15 minutes. Then 30 μL of 10 mmol / L fresh hydroquinone and 520 μL of 3.0 mol / L freshly prepared NaHSO 3 , pH 5.0 (Sigma) were added, and the mixture was incubated at 55° C. for 16 hours. Bisulfite-modified DNA was purified using the Wizard DNA Cleanup kit (Promega). The DNA was desulfonated with NaOH at room temperature for 15 minutes (final concentration: 0.3 mol / L), and neutralized with ammonium acetate at a pH of 7.0 (final concentration: 0.3 mol / L). The DNA was recovered by ethanol precipitation and diluted with distilled water to a final concentration of 5 ng / μL. 3 μL of bisulfite-modified DNA and an equal amount of DNA extracted from 80 μL were...

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Abstract

The invention belongs to the medical field, providing a process for screening colorectal cancer, which screens colorectal cancer through utilizing the hyper-methylation of SFRP 2 gene in feces, and finishes the screening through making specimen, collecting data and treating data. The invention has extreme higher accuracy, and provides a new process for the early discovery and early diagnosis treatment, has reasonable design, free wound, small suffering and low cost, can be a screening process of colorectal cancer, which can increase the curative rate of colorectal cancer at large amplitude if applied in clinics, reduces the fatality rate of colorectal cancer in our country, and has extreme higher economical and social benefits.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular discloses a method for screening colorectal cancer. Background technique [0002] Colorectal cancer refers to malignant lesions of the large intestinal mucosal epithelium under the action of various carcinogenic factors such as environment or genetics, including colon cancer, rectal cancer and anal canal cancer. It is one of the most common malignant tumors of the digestive tract. Bloody stools or mucus pus and bloody stools, changes in stool shape or habit, abdominal pain, abdominal mass, etc. are commonly seen clinically. Depending on the location of its occurrence, its clinical manifestations often have their own specificity. Colorectal cancer has an insidious onset, often has no obvious clinical manifestations in the early stage, and the disease develops slowly, so it is easy to be ignored in the early stage. Risk factors for colorectal cancer include: ①Age over 45 years old, the p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王道荣汤东
Owner 王道荣
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