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Method for preparing sodium alginate/ chitosan sustained-release microcapsule by external gelation

A technology of sodium alginate and calcium chloride chitosan, which is applied in the preparation of microspheres, microcapsule preparations, pharmaceutical formulations, etc. In order to improve the drug content and encapsulation efficiency, narrow particle size distribution and good sphericity

Inactive Publication Date: 2008-08-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] In addition, most of the current methods for removing the residual oil on the surface of the sustained-release microcapsules use organic solvents or surfactants. This method is difficult to completely remove the residual oil on the surface of the sustained-release microcapsules, and a large amount of oil contained in the sustained-release microcapsules will be lost. substances or affect the activity of inclusions

Method used

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  • Method for preparing sodium alginate/ chitosan sustained-release microcapsule by external gelation

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Effect test

Embodiment 1

[0019] Disperse 100ml of doxorubicin (mass concentration of 1.5%) sodium alginate (mass concentration of 1.5%) physiological saline solution into 500ml of edible vegetable oil containing 0.5% span 80, adjust the motor speed to 400r / min and stir for 15 minutes. Add 500ml of 1% calcium chloride and 0.5% chitosan (the degree of deacetylation is 95.3%) to initiate the gelation reaction of sodium alginate. After continuing to stir for 30 minutes, the degreasing device adopts the sedimentation self-floating method to thoroughly remove the alginate. Release the oil on the surface of the microcapsule. Collect and dry naturally to obtain doxorubicin sodium alginate chitosan sustained-release microcapsules. According to "The Pharmacopoeia of the People's Republic of China" 2005 edition appendix VA (appendix 28) ultraviolet-visible spectrophotometry, measure the drug load of doxorubicin in sodium alginate / chitosan sustained-release microcapsules at 481nm, record The content of doxorubic...

Embodiment 2

[0023] Disperse 10ml of sodium alginate normal saline solution with a mass fraction of 3% into 80ml of edible vegetable oil containing 0.5% span80, adjust the motor speed to 1000r / min and stir for 20 minutes. Adding mass concentration is respectively 10% calcium chloride, 1% chitosan (deacetylation degree is 85.7%) solution 50ml causes sodium alginate gelation reaction, after continuing to stir for 60 minutes, collect slow release microcapsule, pass The deoiling device adopts the sedimentation self-floating method to completely remove the oil on the surface of the sustained-release microcapsules, collects, and naturally dries to obtain the sustained-release microcapsules.

Embodiment 3

[0025] Disperse 10ml of sodium alginate normal saline solution with a mass fraction of 1% into 30ml of edible vegetable oil containing 1% span 80, adjust the motor speed to 500r / min and stir for 15 minutes. Adding mass concentration is respectively 3% calcium chloride, 0.5% chitosan (deacetylation degree is 98.2%) solution 50ml causes sodium alginate gelation reaction, after continuing to stir for 20 minutes, collect slow-release microcapsule, pass The deoiling device adopts the sedimentation self-floating method to completely remove the oil on the surface of the sustained-release microcapsules, collects, and naturally dries to obtain the sustained-release microcapsules.

[0026] The degreasing device described in the above-mentioned embodiments 1-3 is shown in Figure 1-3, and it is made up of degreasing tank 1, sedimentation tank 2 and oil collection tank 3, and its height is set from high to low from inside to outside, In the degreasing pool 1, there is a rotating column 4 and...

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Abstract

The invention discloses a method for emulsionizing outer gelation and preparing sodium alginate / chitosan slow release micro-capsule in one step. The current preparation method of calcium alginate gel by internal gelation has defects that small parts of the calcium salt granules cannot be richly reacted with glacial acetic acid and remained in the sodium alginate calcium beads, the encapsulization materials are easily lost and the wrapping rate is low due to the calcium salt is difficult to evenly disperse. The invention uses the sodium alginate liquor as the disperse phase, the edible plant oil as the disperse medium and the sorbitan monooleate as the surfactant to emulsionize small liquid drops wrapped with oil; calcium chloride chitosan liquor is used for initiating the gelation reaction of the sodium alginate so as to generate sodium alginate / chitosan slow release micro-capsule; the oil remained on the surface is eliminated by a oil removing device with precipitation self-drifting method. The invention elevates the micro-capsule medicine contents and the wrapping rate.

Description

technical field [0001] The invention discloses a method for preparing sodium alginate / chitosan sustained-release microcapsules by one-step emulsification and external gelation. Background technique [0002] Sodium alginate / chitosan sustained-release microcapsules have good biocompatibility and mechanical strength, and are an excellent sustained-release drug delivery system, widely used in drug-controlled release carriers, gene delivery vehicles, immune isolation and artificial organs , cell culture microreactors, environmental purification and other aspects. At present, the preparation methods of sodium alginate / chitosan sustained-release microcapsules mainly include spraying method and emulsification method. Spray method mainly adopts coaxial air jet device and electrostatic titration device. The microcapsules prepared by this method have larger particle size, poorer microcapsule sphericity and smoothness, and the particle size of slow-release mic...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/36B01J13/06
Inventor 范春雷沃兴德寿旭锋曲建全
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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