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Chlorpromazine ELISA reagent kit and its detection method

An enzyme-linked immunosorbent reagent, chlorpromazine technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of high cost, cumbersome and time-consuming processing, complicated operation, etc., and achieve the effect of high accuracy, simple structure, and simplified steps

Active Publication Date: 2012-09-05
CHINA JILIANG UNIV +1
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Problems solved by technology

[0004] In order to solve the technical defects of cumbersome and time-consuming sample processing, high cost and complicated operation of the above-mentioned chromatographic technology

Method used

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  • Chlorpromazine ELISA reagent kit and its detection method
  • Chlorpromazine ELISA reagent kit and its detection method
  • Chlorpromazine ELISA reagent kit and its detection method

Examples

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Embodiment Construction

[0019] The methods in the following examples are conventional methods unless otherwise specified.

[0020] 1. Antigen Preparation

[0021] Dissolve 40mg of carrier protein in 1mL of water, take 10mg of chlorpromazine hapten and 30mg of carbodiimide, dissolve in 1mL of water, add the mixture dropwise to the carrier protein solution, and stir overnight at 20-25°C , add 15mg of carbodiimide again, and put it in a refrigerator at 4°C for 24-28h. After the reaction is completed, put the reaction solution into a dialysis bag, dialyze it in PBS with pH=7.4 at 4°C, aliquot and store at -20°C ; Chlorpromazine-BSA as immune antigen.

[0022] 2. Preparation of Polyclonal Antibody

[0023] Immune rabbits:

[0024] New Zealand white rabbits were selected as immunized animals, and 5-10 mL of blood was collected from the ear vein before immunization as a negative serum control. For primary immunization, mix 0.5 mg of immune antigen with an equal amount of Freund's complete adjuvant, and ...

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Abstract

The invention relates to an ELISA Kit and a detection method for detecting chlorpromazine in the veterinary medicine residual detection analysis technical field. The chlorpromazine ELISA Kit comprises a chlorpromazine standard solution, a developer, a concentrated cleaning solution, a termination solution and a sample diluent. In addition, the chlorpromazine ELISA Kit also comprises a chlorpromazine specificity antibody, an enzyme label plate and an enzyme label, wherein the enzyme label is an enzyme labeling chlorpromazine antigen. The ELISA Kit for detecting chlorpromazine provided by the invention has low requirements on the preliminary treatment of a sample, a simple sample preliminary treatment process and a capability of detecting a large number of samples quickly. The ELISA Kit fordetecting chlorpromazine which is characterized in high specificity, high sensitivity, high precision, high accuracy, etc. will contribute much to the detection of chlorpromazine residual.

Description

technical field [0001] The invention relates to an ELISA kit for detecting chlorpromazine and a detection method thereof in the technical field of detection and analysis of veterinary drug residues. Background technique [0002] Chlorpromazine is a representative drug of phenothiazines, a blocker of central dopamine receptors, and has various pharmacological activities such as antipsychotic, antiemetic, cooling and enhancing hypnotic anesthesia. Use during animal feed and transportation can reduce animal maintenance needs and reduce weight loss and mortality on the way. In recent years, some feed companies have randomly added sedative and hypnotic drugs to the feed in order to pursue the conversion rate of feed and high profits. The residues in animal tissues lead to unsafe animal products. In the second half of 2002, in the results of national feed and feed additive quality supervision and spot checks, a total of 258 batches of samples were detected with prohibited drugs a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
Inventor 张明洲王旻子刘军陈宗伦胡华军傅小伟程晔魏建良
Owner CHINA JILIANG UNIV
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