Method of preparing pig replication and respiration syndrome deactivation concentrated vaccine 'SD1 strain'

Abstract

The invention provides a preparation method of an inactivated and concentrated vaccine 'SD1 strain' for a Porcine reproductive and respiratory syndrome, which is prepared with a water phase and an oil phased according to the following weight percentage content: the water phase is prepared by fully mixing 96 shares of 'SD1 strain' virus culture solution, which is American Porcine reproductive and respiratory syndrome that is inactivated for 20 hours and concentrated 2 times, with 4 shares of Tween minus 80, which occupies 33 percent of the vaccine; the oil phase is prepared by mixing 94 shares of No. 10 white oil with 6 shares of Span minus 80 and then adding a 2 percent aluminum stearate according to the total amount to stir to be transparent, and sterilizing with a high pressure at a temperature of 116 DEG C, which occupies 67 percent of the total amount of the vaccine. The vaccine, with a preservation period of 12 months, is safe and reliable to the Porcine reproductive and respiratory syndrome easily infected animals, and is suitable for pigs of different species and various day old, the immunity protection rate of which reaches 80 percent above, the immunity period of validity of which continues more than 6 months. The safety and immunity efficacy of the vaccine reaches an advanced level among the similar products in the world.

Description

1. Technical field [0001] The invention relates to the field of porcine reproductive and respiratory syndrome vaccines in veterinary biological products, in particular to a preparation method of porcine reproductive and respiratory syndrome inactivated concentrated vaccine "SD1 strain". 2. Background technology [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "PRRS", is a new viral infectious disease of pigs that emerged in the late 1980s. The pathogenic PRRS virus (PRRSV) is an enveloped single-stranded positive-sense RNA virus with a genome length of about 15Kb and 9 open reading frames (ORFs). PRRSV can infect pigs of all ages, resulting in decreased semen quality of breeding pigs, miscarriage, return to oestrus, stillbirth or weak piglets, death of piglets and respiratory symptoms of pigs of various ages. PRRS has spread all over the world and has become one of the most serious diseases that endanger the pig industry at home and abroad. ...

AI Technical Summary

Problems solved by technology

[0004] Selection pressure has led to large genetic and antigenic variation of PRRSV epidemic strains in different regions of my country in recent years, and PRRS has not been effectively controlled in some regions

Vaccine immunization is one of the active and effective measures to prevent PRRS. At present, PRRS vaccines include two types of attenuated vaccine and inactivated vaccine. The attenuated vaccine is controversial because of its safety. Denmark once caused a large-scale outbreak of PRRS due to the use of attenuated vaccine. , causing severe economic losses

Although ordinary inactivated vaccines are relatively safe, they are often unable to exert effective immune protection due to insufficient antigen content