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Collagen peptide of Jellyfish with anti-fatigue active and preparation technique thereof

A technology of anti-fatigue activity and collagen, applied in the preparation method of peptides, medical preparations containing active ingredients, medical raw materials derived from cnidaria, etc., to achieve significant anti-fatigue activity and high yield effect

Inactive Publication Date: 2008-08-06
张丽丽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using jellyfish as raw material, there is no report on the preparation of collagen active peptides from jellyfish.

Method used

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  • Collagen peptide of Jellyfish with anti-fatigue active and preparation technique thereof
  • Collagen peptide of Jellyfish with anti-fatigue active and preparation technique thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Take 1000g of commercially available hemp stingers marinated with three alum, drain the water, soak in 2-5 times the amount of deionized water at room temperature, and change the water every 2-3 hours until the content of sodium chloride in the soaking water is low Up to 1.5ppm (chloride ion test was negative). Add 10 g of trypsin with an activity of 4000 Units to the soaked desalted jellyfish, heat and melt at 30-60° C. for 4-24 hours to obtain a suspension. Then centrifuge (4°C, 8000rpm, 30min), discard the insoluble residue, and obtain a clear and transparent liquid, which is the collagen active peptide liquid; the collagen active peptide liquid is placed on a rotary evaporator, concentrated at 40°C, 0.001MPa to the original 1 / 5 of the volume is then spray-dried (inlet air temperature 185° C., outlet air temperature 85° C.) to obtain 20 g of collagen active peptide powder solid with a purity of more than 90% and significant anti-fatigue activity.

example 2

[0013] Get 1000g of fresh jellyfish, soak in 2-5 times the amount of deionized water at room temperature, and change the water every 2-3 hours until the content of sodium chloride in the soaking water is lower than 1.5ppm (the chloride ion test is negative ). Add 10 g of trypsin with an activity of 4000 Units to the soaked desalted jellyfish, heat and melt at 30-60° C. for 4-24 hours to obtain a suspension. Then centrifuge (4°C, 8000rpm, 30min), discard the insoluble residue, and obtain a clear and transparent liquid, which is the collagen active peptide liquid; the collagen active peptide liquid is placed on a rotary evaporator, concentrated at 40°C, 0.001MPa to the original 1 / 5 of the volume is then freeze-dried to obtain 10 g of solid collagen active peptide powder with a purity of more than 95% and significant anti-fatigue activity.

example 3

[0015] A sample of collagen active peptide powder was tested for protein content, total sugar content, amino acid composition and monosaccharide composition. The measurement results showed that the sample contained 88% protein, 7.5% sugar, and the average molecular weight was 1274. The monosaccharide contained is mainly glucose, and the composition of amino acids contained in it is shown in Table 1.

[0016] Table 1 Amino acid composition of test samples

[0017]

[0018]

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PUM

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Abstract

The invention discloses a collagen activity peptide of jellyfish source with anti-fatigue action and a preparation technique thereof, belonging to a field of natural bioactive substances and the preparation technology thereof, which provides a novel natural marine bioactive peptide. The operation according to the preparation technique can lead the collagen activity peptide of 2 percent purity to that of 90 percent above purity. The novel collagen activity peptide has no toxic and side effects at all and has remarkable anti-fatigue action.

Description

technical field [0001] The invention relates to a collagen active peptide with anti-fatigue activity derived from jellyfish and a preparation process thereof, belonging to the technical field of natural bioactive substances and their preparation. Background technique [0002] Cyanea nozakii, commonly known as hemp sting, is a large marine plankton belonging to Phylum Cnidaria, Class Scyphomedusae, Order Phylum cnidaria, and family Jellyfish. (Family Cyanea). There are 4 species of white rosy jellyfish (Cyanea nozaki Kishinouye), hair-shaped rosy jellyfish (Cyanea capillata), brown rosy jellyfish (Cyanea ferruginea Eschscholtz), and purple rosy jellyfish (Cyanea purpurea Kishinouye). The largest number and the widest distribution range. The jellyfish usually uses small zooplankton as bait. It has well-developed gonads, strong reproductive ability, and extremely fast growth. At present, the annual output of jellyfish in my country has reached 10 million tons, forming a new n...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61P43/00C07K1/14C07K14/78C12P21/02A61K35/614
Inventor 汤鲁宏张本田姜瑞霞
Owner 张丽丽
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