Method for extracting and separating meletin from Mesona Procumbens Hemsl

Abstract

The invention discloses a method for extracting quercetin from all-grass of grass jelly, which comprises the following steps: (1) getting a plurality of all-grass of grass jelly, adding 20 times of water to boil and extract, slowly adding limewater during agitation until the pH is 8 to 9, then adding 3 percent of a boric acid and boiling for 20 to 30 minutes under the pH condition; (2) filtering before getting cold, repeatedly depressurizing and filtering the residue 3 times and combining the filtrate, under the condition of 60 to 70 DEG C, adjusting a combined liquid to pH value of 5 by concentrated hydrochloric acid, pumping the acid liquid after placing for 24 hours; (3) washing the precipitate to neutrality by water, drying in 60 DEG C to derive a rutin crude product, recrystallizing by boiling water, and drying in 70 to 80 DEG C to derive the rutin pure product; (4) adding 2 percent of H2SO4 to the refined rutin by a proportion of 1:80, heating with fire for 2 hours, cooling and pumping; (5) washing the residual water to neutrality, and then implementing 95 percent ethanol recrystallization and sephadex chromatography separation (SphadexLH20), using carbinol as an eluant; (6) deriving quercetin yellowish green pure product, as the rutin can not be eluted. The invention has the advantages of simplicity, low cost and high yield, thus being suitable for industrial production.

Description

Technical field: [0001] The invention discloses a method for extracting and separating quercetin from plant jellyfish. Background technique: [0002] Grass jelly, also known as jelly grass, fairy grass, fairy jelly, fire grass, etc., is an annual herb of the Lamiaceae genus Xiancao. Dried grass jelly contains about 70% carbohydrates, a small amount of protein, fat, pigment, etc., and more mineral elements. Among the functional components contained in Grass jelly, ursolic acid can not only reduce blood fat, but also lower liver fat. It has the functions of expectorant and cough relief, and has a certain antiasthmatic effect. In addition, it also has the effects of lowering blood pressure, enhancing capillary resistance, reducing capillary fragility, lowering blood fat, expanding coronary arteries, and increasing coronary blood flow. It is used for the treatment of Chronic bronchitis also has adjuvant therapeutic effect on patients with coronary heart disease and hypertensio...

AI Technical Summary

Problems solved by technology

Quercetin, which is insoluble in water, has good expectorant and cough-relieving effects, and has a certain anti-asthmatic effect exists in the jelly jelly dregs after the jelly jelly is extracted and is discarded as waste, resulting in a huge waste of resources

At present, there is a lack of systematic research on the extraction of quercetin from grass jelly