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Immune bovine colostrum and method for preparing bifidobacteria composite tiny capsule and pill

A bifidobacterium and colostrum technology, applied in the direction of medical preparations containing active ingredients, sugar-coated pills, pill delivery, etc., can solve the problems of reducing the activity, quantity and potency of immune bovine colostrum and bifidobacteria, and achieve Good release effect in the intestinal tract, strong acid resistance and anti-digestive enzyme properties, high immunity and micro-ecological regulation dual activity effect

Inactive Publication Date: 2008-07-16
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for preparing composite microcapsules by vacuum freeze-drying, suspension granulation and enteric coating after fermenting immune bovine colostrum by using bifidobacteria, aiming to solve the problem of immune bovine colostrum and immune bovine colostrum in the process of production and use. The problem of significant reduction in the activity, quantity and titer of bifidobacteria

Method used

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  • Immune bovine colostrum and method for preparing bifidobacteria composite tiny capsule and pill
  • Immune bovine colostrum and method for preparing bifidobacteria composite tiny capsule and pill

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of bifidobacterium fermented immune bovine colostrum

[0028] 1. Preparation of immune bovine colostrum

[0029] 1. Antigen preparation

[0030] Shigella flexneri (Shigella flexneri, purchased from the Microbiology Laboratory of the First Clinical Hospital of Jilin University, conventional strains) was enriched and cultured at a shaking table temperature of 37°C and a rotational speed of 200rpm for 18-20 hours; the culture solution was collected and Add 0.4% formaldehyde at a volume concentration, inactivate at 37°C for 24 hours, and perform miscellaneous inspection. If there is no viable bacteria, use a McFarland tube to measure the bacterial concentration. Adjust the cell concentration to 1.0×10 with sterile normal saline 11 cfu / mL to make water-based seedlings, and add an equal volume of No. 10 white oil to the water-based seedlings, stir and emulsify to make oil-adjuvanted seedlings; both water-based seedlings and oil-adjuvanted seedlings a...

Embodiment 2

[0051] Example 2: Preparation of immunized bovine colostrum and bifidobacterium composite freeze-dried powder

[0052] Add 1% mass concentration of sucrose and 4% mass concentration of mannitol in bifidobacterium fermented immunized bovine colostrum as lyoprotectant. The temperature of the cold trap is -45°C, and the thickness of the feed liquid is 10mm. First, it is pre-frozen at -30°C for 2 hours; The temperature was controlled below 20°C, and the freeze-drying time was 10 hours. The number of viable bacteria in the compound freeze-dried powder was 1.24×10 10 cfu / g dry powder, immunoglobulin titer 2 9 .

Embodiment 3

[0053] Example 3: Preparation of immune bovine colostrum and bifidobacterium composite microcapsules

[0054] 1. Production of mother core

[0055] The mother core is made of sucrose powder and soluble starch with a mass ratio of 7:3. The working parameters of the mother core granulator are: host speed regulation: 80rpm, spray speed regulation: 110rpm, blast temperature: 27.1°C.

[0056] 2. Drying of the mother core

[0057] After the master core is preliminarily shaped and dried, the unqualified master batches are removed, and the drying temperature is 40°C, the relative humidity of the air is ≤20%, and the drying time is 4 hours. The average weight of the mother core is 0.226mg, and the diameter is 0.3-0.5mm.

[0058] 3. Preparation of immunized bovine colostrum and bifidobacterium composite freeze-dried powder particles

[0059] The immune bovine colostrum and bifidobacterium composite freeze-dried powder were coated on the mother core by air suspension granulation metho...

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Abstract

The invention relates to a preparation method of a compound micro-capsule of hyper-immune bovine colostrum and bacillus bifidus which inoculates bacillus bifidus (inoculum concentration: 1-10 percent ) to degreased hyper-immune bovine colostrum that goes through ultra-high pressure sterilization and cultures for 4-24h to acquire bacillus bifidus fermented hyper-immune bovine colostrum; freeze-dry protecting agent is added and vacuum cryodesiccation is carried out, and lyophilized powder of hyper-immune bovine colostrum and bacillus bifidus is obtained; a mother core and compound lyophilized powder with a mass ratio of 1: 4-14 are prepared into lyophilized powder of hyper-immune bovine colostrum and bacillus bifidus via air suspension granulation method, then enteric soluble opadry coating liquid at a concentration of 5-50 percent is adopted as embedding medium for enteric coating during air suspension granulation to have the compound micro-capsule prepared. The acquired compound micro-capsule can stand the killing of gastric acid, remain immune effectively and micro-ecological double reactive, reduce the loss of activity, quantity and potency of hyper-immune bovine colostrum and bacillus bifidus during the production and usage process of the product and enhance health care function of the product to human body.

Description

technical field [0001] The invention relates to a method for preparing composite microcapsules with good immunity and microecological regulation dual activities through vacuum freeze-drying, suspension granulation and enteric coating after fermenting immunized bovine colostrum by using bifidobacteria. Background technique [0002] At the end of the 19th century, humans began to recognize bifidobacteria and immune milk. After more than a century of research and practice, human beings have understood and mastered the basic characteristics of the two, and have developed a variety of products. [0003] Bifidobacterium is the main beneficial flora in the human intestinal tract. It can inhibit the growth and reproduction of harmful flora, promote the body's metabolism, enhance the body's resistance, and regulate the micro-ecological balance in the human body. It has been used in health care drugs and food. Bifidobacterium products are very popular in countries such as Japan, Euro...

Claims

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Application Information

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IPC IPC(8): A61K35/20A61K35/74A61K9/28A61P31/04A61K35/745
Inventor 刘景圣
Owner JILIN AGRICULTURAL UNIV
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