Clone and application of molecule mark used as swine mark auxiliary selection and correlated with immunity

A marker-assisted selection and molecular marker technology, applied in the field of livestock genetic engineering, can solve problems such as the function of pig BCL10 gene that has not been fully studied.

Inactive Publication Date: 2008-07-09
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] So far, there has been no comprehensive study on the function of the pig BCL10 gene, but the study of the polymorphism of the mutation site in the population and the association analysis of traits are a very powerful means to study the function of the gene

Method used

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  • Clone and application of molecule mark used as swine mark auxiliary selection and correlated with immunity
  • Clone and application of molecule mark used as swine mark auxiliary selection and correlated with immunity
  • Clone and application of molecule mark used as swine mark auxiliary selection and correlated with immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) BCL10 gene CDS cloning and partial DNA sequence amplification

[0035] (1) Primer design

[0036] Use human BCL10 gene mRNA (GenBank accession number: NM 003921) as an information probe, utilize the BLAST tool in NCBI to do homologous sequence screening in the GenBank pig EST database, and obtain a series of ESTs with more than 90% homology (fragment length greater than 100bp), use ENTREZ (http: / / www.ncbi.nlm.nih.gov / Web / Search / index.html) to query the corresponding sequences of these ESTs in NCBI, and then use the sequence analysis software DNAStar The SeqMan program constructs porcine EST-contigs. According to the homologous comparison with the human BCL10 gene mRNA, the start and stop codon positions were preliminarily determined, and primers were designed to clone the cDNA sequence (including the full-length CDS and part of the 3'-UTR) as shown in SEQ ID NO: 1 in the sequence table According to the result of comparison with the full-length DNA sequence of the ...

Embodiment 2

[0062] The experimental pigs came from the Landrace pigs of the Shuitai original pig farm of Guangdong Huanong Wenshi Animal Husbandry Co., Ltd. (the blood of foreign pig breeds). The parents were 17 Landrace boars and 36 Landrace sows. The first piglets, parents and offspring were all genotype tested. The offspring collected anticoagulant and non-anticoagulant blood at 0, 17, and 32 days of age, and routine blood routine and antibody levels were detected by conventional methods.

[0063] The traits analyzed are mainly immune traits, including porcine reproductive and respiratory syndrome (PRRS) virus antibody levels, classical swine fever virus antibody levels, pseudorabies antibody levels and 18 blood routine items. According to the population structure of the collected samples, the applicant used a mixed model to statistically analyze the genotype effect of the SNPs of the BCL10 gene and its relationship with immune indicators:

[0064] Y=Xβ+Zγ+e

[0065] Among them, Y is ...

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Abstract

The invention belongs to the technical field of the livestock genetic engineering and in particular is a molecular marker which is used as the application to pig marker-assisted selection and relative with immune traits and the application. The molecular marker is obtained by cloning gene BCL10, the nucleotide sequence of the molecular marker is displayed as the sequence table SEQ ID NO: 2, and the number 144 basic group position of the sequence which is displayed in the sequence table SEQ ID NO: 2 has a gene mutation of C144-T144, which leads to the polymorphism of the RFLP-HinP1I. The invention also discloses a process for preparing the molecular marker and the application of a polymorphism testing method and provides a new molecular marker for the pig marker-assisted selection.

Description

technical field [0001] The invention belongs to the technical field of livestock genetic engineering, and in particular relates to the cloning and application of a molecular marker related to immune traits used as pig marker-assisted selection. Background technique [0002] In recent years, modern breeding techniques have made great contributions to improving pig production traits, but genetic improvement of health and disease resistance has not received enough attention. The application of some preventive measures, such as improved management, improved sanitation, injection of drugs and vaccines, etc., have played an important role in modern pig farming, but as the global call for reducing the use of drugs and vaccines is getting louder, The use of molecular genetics methods to essentially improve the disease resistance of livestock and poultry organisms has attracted more and more attention and has become the focus of research. [0003] Disease resistance is generally coo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/10C12Q1/68
Inventor 赵书红黄菁余梅朱猛进李长春李新云
Owner HUAZHONG AGRI UNIV
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