Method for producing insect parasitic bacteria fruit body

Abstract

The invention provides a method for rapidly and stably producing fruiting body of insect parasite. The method comprises the following steps of: injuring epidermis of an insect, inoculating an insect parasite at the injured section, and culturing the insect parasite in the insect body to obtain the fruiting body of the insect parasite. The insect parasite can be selected from, such as, Cordyceps sinensis, and the insect is selected from Lepidopteran insect such as Spodoptera litura. Preferably, the insect parasite is cultured in an agar culture medium or a grain culture medium to generate mycelia of the insect parasite and is inoculated by disposing the insect with injured epidermis on the insect parasite. Additionally, the insect parasite can propagate in the insect body, so that the insect is hardened and the fruiting body is generated using the portion of the epidermis where a fruiting body primodium is generated as the seed.

Description

technical field [0001] The present invention relates to a method for rapidly and stably producing fruiting bodies (mushrooms) of insect parasitic fungi such as Cordyceps sinensis fungi, which are utilized as health food, pharmaceuticals, cosmetics, etc. Background technique [0002] Fruiting bodies of insect parasitic fungi, such as Cordyceps sinensis, can be used as traditional Chinese medicine and high-grade food materials. The fruiting bodies of Cordyceps sinensis fungi are usually picked and grown naturally, so the number is often small, the size is different, and the quality is not stable. In addition, even if the insect parasites are of the same genus as Cordyceps militaris, they often have differences in growth rate, fungal growth and entomopathogenicity. Therefore, people have been exploring excellent insect parasites and studying their artificial culture methods. [0003] Specifically, it has been proposed that host insects are artificially inoculated with Cordyce...

AI Technical Summary

Problems solved by technology

[0007] In addition, in the methods described in Japanese Patent Laid-Open No. 10-42691 and International Publication No. WO01 / 022801 pamphlet, it is also difficult to generate ascospores, and it is also difficult to ensure a stable ascospore as an inoculum source.

In other words, these methods have the following problems: insect parasites such as Cordyceps sinensis fungi produce less fruiting bodies, and in dry years, they are hardly seen even in the wild, and it is difficult to ensure stable production.

At the same time, even if the fruiting bodies are picked in the wild, it is difficult to obtain ascospores with the same stable quality and not contaminated by bacteria

For example, even if a suspension made of such naturally occurring ascospores is injected into the body of insects such as larvae or pupae to infect them, there are still a large number of individuals corrupted by the bacteria, and the yield becomes low and the stability cannot be stabilized. Problems like producing child entities

Images