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Preparations for treating mastitis of cow and preparation thereof

A technology for dairy cow mastitis and preparations, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, peptide/protein components, etc., can solve the problems of increasing drug-resistant strains, aggravating drug residues, and increasing the dosage of antibiotics. The effect of slowing down the degradation rate, prolonging the curative effect, and prolonging the action time of the drug

Inactive Publication Date: 2008-07-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long-term, extensive, and high-dose use of antibiotics to treat dairy cow diseases has led to a series of adverse consequences
The first is the problem of food public health: after treatment with antibiotics, there is a potential risk of drug residues in milk, which will adversely affect human health; secondly, the increase in drug-resistant strains: pathogenic bacteria develop drug resistance, making mastitis more severe It is becoming more and more difficult to cure, and veterinarians on cattle farms have to increase the dosage of antibiotics, which increases the risk of drug residues; other hazards of drug residues include inhibition of the fermentation process in dairy production, resulting in reduced product yield and quality
When we used this preparation to treat cow mastitis, we found that the degradation rate of nisin in mastitis milk was faster than that in normal milk, and the antibacterial potency would drop significantly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1EDTA-Na 2 Prolonging the action time of lactococcus as a protective agent of lactococcus in milk with mastitis

[0018] (1) Select dairy cows that have not been treated with antibiotics for one month and have just developed clinical mastitis in the dairy farm, and collect 350ml of milk from the diseased dairy area.

[0019] (2) Centrifuge the milk at 3500 rpm for 20 minutes, remove the fat in the upper layer, and divide the skim milk into 3 Erlenmeyer flasks, each with 100 ml.

[0020] (3) Add 100,000 IU / 100ml of lactococcus into each Erlenmeyer flask, and then add 400μl of 0.5mol / L EDTA, mix well and place in a water bath at 37°C for 12 hours.

[0021] (4) After the water bath, refer to the national standard GB2760-86 nisin titer test method to detect the antibacterial effect of 3 bottles of skim milk (containing nisin, nisin+EDTA and EDTA) on Micrococcus luteus Effect, the size of the antibacterial effect is represented by the diameter of the inhibition zon...

Embodiment 2

[0026] (1) 200 grams of vitamin C (product of Guangdong Chemical Reagent Engineering Technology Research and Development Center) and 139 grams of nisin (product of Zhejiang Yinxiang Biological Engineering Co., Ltd.) , and 800 g EDTA-Na 2 mix well;

[0027] (2) The mixed powder in (1) is divided into 1000 sterilized glass ampoules with a capacity of 20 milliliters in 1.14 grams per portion, and the product is obtained.

[0028] (3) Inject 20 ml of normal saline into an ampoule and shake it to fully dissolve the drug. The pH of the solution is 3, the titer of nisin is 125,000 units / ml, vitamin C 10 mg / ml, EDTA-Na 2 40mg / ml.

Embodiment 3

[0030] (1) 200 grams of vitamin C (product of Guangdong Chemical Reagent Engineering Technology Research and Development Center) and 125 grams of nisin (product of Zhejiang Yinxiang Biological Engineering Co., Ltd.) with a titer of 20000 units / mg were aseptically processed , and 500 g EDTA-Na 2 mix well;

[0031] (2) The mixed powder in (1) is divided into 1000 sterilized glass ampoules with a capacity of 20 milliliters in 0.83 grams per portion, to obtain the final product.

[0032](3) Inject 20 ml of normal saline into an ampoule and shake it to fully dissolve the drug. The pH of the solution is 3, the titer of nisin is 125,000 units / ml, vitamin C 10 mg / ml, EDTA-Na 2 40mg / ml.

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PUM

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Abstract

The invention relates to a preparation for the treatment of dairy cow mastitis and the preparation method thereof, the preparation is a water or physiological saline solution containing nisin, vitamin C and EDTA-Na2; each milliliter of water or physiological saline solution contains 0.05 to 0.125 million units of nisin, 4 to 10 milligrams of vitamin C and 16 to 40 milligrams of EDTA-Na2. The nisin, vitamin C and EDTA-Na2 sterile powder with the proportion are mixed and packaged, and the sterile powder is dissolved in water or physiological saline before use. The experiments prove that the medicament can inhibit the activity of protease in milk after the EDTA-Na2 is added, so as to slow the degradation speed of nisin in milk, prolong the action time of the drug and prolong the efficacy for the treatment of dairy cow mastitis.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a pharmaceutical preparation for treating cow mastitis and a preparation method of the preparation. Background technique [0002] Mastitis is one of the most serious diseases in dairy farming. About 50% of dairy cows in the world suffer from various types of mastitis. Cows suffering from mastitis have reduced milk production and quality. The direct economic loss caused by cow mastitis in the United States reaches 2 billion US dollars every year. About 70% of the loss caused by mastitis in dairy cows is the decline in milk production caused by recessive mastitis. In China, the annual loss caused by recessive mastitis alone is at least 135 million yuan. The main cause of mastitis in dairy cows is the infection of pathogenic microorganisms. Therefore, the main drug for the treatment of mastitis at present is antibiotics. The long-term, extensive, and high-dose use of antibiotics to t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P15/14
Inventor 胡松华
Owner ZHEJIANG UNIV
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