Outer test-tube rooting method for aralia mandshurica tissue culture seedling

Abstract

The invention belongs to the technical field of plant cultivation, relating to a method for rooting an aralia elata seem by a tissue culture seedling outside of a test tube. The invention is characterized in that the invention carries out a treatment on the tissue culture proliferous seedlings not entering into rooting cultivation during an aralia elata seem tissue culture process and then roots outside of the test tube. The detail steps are as follows: 1) fetching the aralia elata seem tissue culture proliferous seedlings; 2) treating before rooting; 3) rooting outside the test tube; 4) managing the rooting seedlings. The invention uses no growth regulators during the rooting process and can effectively avoid a sensitive reaction of the aralia elata seem tissue culture seedlings on cultivation growth regulators, thus reducing rooting difficulties. The invention realizes the fast rooting of the aralia elata seem tissue culture seedling outside the test tube and carries out technical improvements on tissue culture and fast breeding, thus leading the rooting rate and survival rate to be improved greatly.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to the cultivation of wild plant tissue culture seedlings, in particular to a method for rooting out of test tubes of tissue culture seedlings of Aralia japonica. Background technique [0002] Aralia elata (Miq.) Seem.], also known as magpie not stepping, thorn old bud, Liaodong Aralia, is a plant of the genus Aralia Linn. in the Araliaceae family. Deciduous shrubs and small trees, 1.5-6.0m high, growing in miscellaneous forests, broad-leaved forests or mixed coniferous and broad-leaved forest margins and shrubs below 1000 meters above sea level, mostly on hillsides and river valleys. Distributed in Northeast China, North Korea, Japan, and the former Soviet Union. The main parts used in traditional Chinese medicine are root bark and bark; in addition, the young shoots of Aralia japonica are called thorny shoots, which are rich in nutrition and are popular wild vegetables. ...

AI Technical Summary

Problems solved by technology

[0003] A large number of studies have shown that in terms of seedling raising methods, Zhang Meiping et al. (1998) adopted the method of sowing seedlings with seed seeds for cultivation. Although the seedlings grow quickly, because they are collected from wild natural hybrid populations, their seed properties are unstable and the offspring have great variability. Large, severe differentiation, so that the species are mixed and difficult to control; Tian Hong et al. (1998) used the method of transplanting wild root tiller seedlings for artificial cultivation. This method belongs to asexual reproduction, and the species is stable, but insufficient resources limit large-scale Production; there are many reports on rapid propagation by tissue culture, Na Ningxin (2002), Chen Guoshuang, Shao Qingchun (2003), Li Jianmin (2004), Ran Xuezhong (2005) etc. utilize tissue culture methods to carry out rapid propagation, although the cost is relatively high relative to seed propagation High, but can maintain the good traits of the female parent, which is of great significance for the rapid propagation of new good varieties (lines); there are also direct propagation of seedlings by cuttings, but the survival rate is low

[0004] It is a feasible technology to use tissue culture method to carry out asexual rapid propagation of excellent mother plants. However, due to the different culture sites and exogenous hormone types used by domestic scholars in the tissue culture process, the results obtained are not the same. It may also be due to the different genotypes or ecotypes and parts of the explants; in addition, in most conventional tissue culture processes, the problem of rooting is difficult to varying degrees. elemental approach, often causing poor growth and reduced survival