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Method for rapidly culturing anaerobic ammonium oxidation bacteria by up-flow type anaerobic sludge bed reactor

A technology of anammox bacteria and flow anaerobic sludge, which is applied in the field of anammox bacteria cultivation, can solve the problems of unstable operation of the reactor, slow bacterial growth, long acclimation time, etc., and achieves stable operation, High nitrogen removal rate and good nitrification effect

Inactive Publication Date: 2011-09-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the shortcomings of ANAMMOX bacterial growth such as slow growth, long domestication time, low treatment efficiency, and unstable reactor operation limit the application of anammox technology in wastewater denitrification treatment.

Method used

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  • Method for rapidly culturing anaerobic ammonium oxidation bacteria by up-flow type anaerobic sludge bed reactor

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Experimental program
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Effect test

Embodiment 1

[0023] (a) In the UASB reactor, the biofilm can effectively retain the enriched microorganisms, and the cultivation of the biofilm is conducive to the accumulation of ANAMMOX bacteria. The inoculum sludge is taken from the activated sludge in the leachate SBR process of a landfill site. The sludge is reddish brown. The ordinary optical microscope inspection shows that the sludge structure is finely divided, and there are bell worms, larvae and rotifers. There are few filamentous bacteria, MLSS is 55.63g / L, and the inoculation amount is 65% of the effective volume of the reactor. The SBR process itself has the characteristics of process operation, including anaerobic, anoxic, aerobic and static stages, and sludge When the nitrification effect of the SBR process is good, there are a variety of microorganisms in the inoculated sludge from the aerobic SBR process for treating landfill leachate, which is conducive to accelerating the enrichment of anammox bacteria. Dissolved oxygen...

Embodiment 2

[0027] (a) In the UASB reactor, the biofilm can effectively retain the enriched microorganisms, and the cultivation of the biofilm is conducive to the accumulation of ANAMMOX bacteria. The inoculum sludge is taken from the activated sludge in the leachate SBR process of a landfill site. The sludge is reddish brown. The ordinary optical microscope inspection shows that the sludge structure is finely divided, and there are bell worms, larvae and rotifers. There are few filamentous bacteria, MLSS is 50.23g / L, and the inoculation amount is 60% of the effective volume of the reactor. The SBR process itself has the characteristics of process operation, including anaerobic, anoxic, aerobic and static stages, and sludge When the nitrification effect of the SBR process is good, there are a variety of microorganisms in the inoculated sludge from the aerobic SBR process for treating landfill leachate, which is conducive to accelerating the enrichment of anammox bacteria. Dissolved oxygen...

Embodiment 3

[0031] (a) In the UASB reactor, the biofilm can effectively retain the enriched microorganisms, and the cultivation of the biofilm is conducive to the accumulation of ANAMMOX bacteria. The inoculum sludge is taken from the activated sludge in the leachate SBR process of a landfill site. The sludge is reddish brown. The ordinary optical microscope inspection shows that the sludge structure is finely divided, and there are bell worms, larvae and rotifers. There are few filamentous bacteria, MLSS is 59.67g / L, and the inoculation amount is 70% of the effective volume of the reactor. The SBR process itself has the characteristics of process operation, including anaerobic, anoxic, aerobic and static stages, and sludge When the nitrification effect of the SBR process is good, there are a variety of microorganisms in the inoculated sludge from the aerobic SBR process for treating landfill leachate, which is conducive to accelerating the enrichment of anammox bacteria. Dissolved oxygen...

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Abstract

The present invention discloses a method by using an up-flow anaerobic sludge blanket-biomemebrane reactor to quickly cultivate anaerobic ammonium oxidation bacteria, comprising the following steps: enriched microorganisms are preserved in an internal membrane of a UASB reactor; garbage landfill is selected for treating activated sludge during the leachate SBR process as seed sludge, and seeded sludge quantity is 45 to 65 percent of effective volume of the reactor; a constant temperature circulating water bath is adopted to maintain temperature of a reaction zone to be 1 DEG C higher or lower than32 DEG C; waste water is synthesized by adoption of manpower, and waste water components comprise NH4Cl, NaNO2, MgSO4, KH2PO4, CaCl2, NaHCO3, a microelement I and a microelement II; mass ratio of NH4<+>-N and NO2<->-N during the process of water distribution is controlled to be ranged from 1 to 1.0 to 1 to 1.6; PH value of influent is adjusted to be 7.4 to 7.8. The method of the present invention has the advantages of simple technology, stable system operation, high nitrogen removal rate, short acclimation concentration time and so on.

Description

technical field [0001] The invention relates to the cultivation of anaerobic ammonium oxidizing bacteria, in particular to a method for quickly cultivating anaerobic ammonium oxidizing bacteria in an upflow anaerobic sludge bed reactor. Background technique [0002] With the development of industry and agriculture and the improvement of people's living standards, the pollution of nitrogen has become increasingly serious and has become one of the main factors of water environment pollution. Many countries have increasingly stringent requirements for nitrogen in wastewater discharge standards. Therefore, the research and development of wastewater biological denitrification technology has attracted people's attention. However, traditional biological denitrification technologies generally have defects such as high infrastructure investment and operating costs, complex operation control, long process, high oxygen consumption, and low denitrification effect. Researchers have been...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12M1/38C12R1/01
Inventor 周少奇朱明石
Owner SOUTH CHINA UNIV OF TECH
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