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Porcine circovirus and helicobacter combination vaccines and methods of use

A porcine circovirus and Helicobacter technology, applied in the direction of virus/bacteriophage, virus, vaccine, etc., can solve the problems of microbial flora disorder and other problems

Inactive Publication Date: 2008-06-11
MERIAL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, disturbance of the microflora due to antimicrobials would also be undesirable

Method used

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  • Porcine circovirus and helicobacter combination vaccines and methods of use
  • Porcine circovirus and helicobacter combination vaccines and methods of use
  • Porcine circovirus and helicobacter combination vaccines and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0244] Example 1: Method for Isolating and Characterizing PCV Isolates

[0245] 1) Cell culture: PCV-free PK15 derivative, ie Dulac cell line, was obtained from Dr. John Ellis (University of Saskatchewan, Saskatoon, Saskatchewan). The Vero cell line was obtained from the American Type Culture Collection (ATCC), Manassas, VA. These cells were cultured in medium as recommended by ATCC and incubated at 37 °C and 5% CO 2 incubate.

[0246] Porcine Circovirus: Conventional PCVI was isolated from persistently infected PK15 cells (ATCC CCL33). Isolate PCVII 412 was obtained from piglet lymph nodes challenged with lymph node homogenate from PMWS-affected piglets. This challenged piglet was diagnosed with PMWS. Isolate PCVII 9741 was isolated from the peripheral buffy coat of PMWS-affected piglets from the same herd following the isolation of PCVII 412. Isolate PCVII B9 was isolated from affected piglets in an American herd with a clinical outbreak of PMWS in the fall of 1997. ...

Embodiment 2

[0260] Example 2: PMWS reproduction

[0261] PMWS has not yet reproduced under controlled conditions, nor has its etiology been studied. To determine the causative agent of this disease, a number of tissues were collected from PMWS-affected pigs and studied as described in Example 1 above. Lymph nodes showed the most obvious gross lesions, histopathological changes, and circovirus infection was confirmed by immunostaining. Therefore, lymph nodes were used in the challenge experiments described above.

[0262] Challenge experiments performed as described in Materials and Methods successfully produced PMWS in pigs. In particular, some piglets died from infection and asymptomatic infected piglets developed PMWS-like microscopic lesions at the end of the experiment.

[0263] In another challenge experiment, the starting material used was lung tissue from pigs with chronic wasting and lymphadenopathy. These clinical signs are characteristic of PMWS. The tissue was combined w...

Embodiment 3

[0271] Example 3: Isolation and Propagation of PCVII

[0272] To determine the presence of infectious causative agents for PMWS, various tissues from pig #412 (experimentally challenged piglets sacrificed 21 days after infection) were used for virus isolation. Virus accumulation or adaptation was observed after serial passage of lymph node samples from pig #412 in Dulac cells. A distinct pattern of cytopathic effect initially developed, followed by an increase in virus titer determined by ELISA using standard Berlin anti-PCV antibodies as described above.

[0273] The presence of circovirus in Dulac cells infected with isolate PCVII 412 was subsequently detected by electron microscopy. After 6 passages, viral structural proteins were consistently detectable using Western blot assays.

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PUM

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Abstract

The present invention is based on the discovery of a novel Helicobacter species associated with gastroesophageal ulcers in pigs. In particular, the novel species H. cerdo has been used as a source of antigenic material for the development of vaccines for the treatment of gastroesophageal disorders. Most advantageously, novel Helicobacter and porcine circovirus associated with PMWS in pigs are used to provide a combination vaccine whereby immunogens derived from both pathogen types can be co-delivered to target animals to stimulate protective antibodies and immunity generation. Accordingly, the present invention provides vaccines for the treatment of gastroesophageal ulcers and PMWS in pigs. Thus, the present invention includes multivalent immunogenic compositions and vaccines, multiple vaccine kits, and combined immunization or vaccination methods that enable the use of such combined immunization or vaccination programs.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Application Serial No. 11 / 107,219, filed April 15, 2005, the contents of which are hereby expressly incorporated herein by reference. [0003] This application is a continuation-in-part of pending U.S. Application Serial No. 10 / 653,849, filed September 2, 2003, which is a part of pending U.S. Application Serial No. 10 / 334,245, filed December 31, 2002 Continuation of Abandoned U.S. Application Serial No. 09 / 935,428, Filed August 20, 2001, Abandoned U.S. Application Serial No. 09 / 935,428, Filed December 10, 1998 Continuation of Ser. No. 09 / 209,961, which claims priority to US Application Serial No. 60 / 069,750, filed December 16, 1997, and US Application Serial No. 60 / 069,233, filed December 11, 1997. [0004] This application is also a continuation-in-part of pending U.S. Application Serial No. 09 / 884,514, filed June 19, 2001, which is a divisional application of U.S. Application Seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/34C07K14/01C07K14/205A61K39/295
CPCA61K39/12A61K2039/525C07K16/081A61K2039/552C07K14/005C12N7/00A61K39/105C12N2750/10021A61K2039/5254A61K2039/53A61K39/295C12N2750/10043A61K39/00A61K2039/55566C12N15/86A61K2039/521A61K2039/5256A61K2039/70C12N2710/24043C12N2750/10034
Inventor J·埃里斯C·沙雷尔O·M·马汀昂
Owner MERIAL LTD
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