Preparation method for pig blood vessel acellular bracket by chemical and physical combination

Abstract

The invention relates to a preparation method of a porcine vascular cell-free stent with the combination of chemistry and physics, which mainly includes the steps of material preparation, membrane rupture of superficial cells, removal of superficial cells, removal of deep cells and so on. Surfactant is used for the membrane rupture of deep cells at the same time of completing the removal task of superficial cells, so the incubation and oscillation only need to be carried out for about 1 hour in the surfactant solution with appropriate concentration, and the removal task of the deep cells can be completed by the ultrasonic waves with lower power in a shorter time; the mutual cooperation can allow the porcine vascular cell-free stent to have the advantages of lower equipment cost, shorter preparation time, thorough removal of the cells, easy clean of the surfactant, complete preservation of extracellular matrix (stent), good biocompatibility, appropriate degree of biodegradation rate, high mechanical strength, easy suture and fixation and so on; furthermore, the stent is also conductive to the colonization and growth of human cells and can be gradually degraded and absorbed along with the regeneration of real blood vessel.

Description

technical field [0001] The invention relates to a medical material, in particular to prepare pig blood vessels as substitute materials for human blood vessels. The invention belongs to the field of medical prosthesis materials. Background technique [0002] Complex heart and great vessel malformations often require the use of biological blood vessels. At present, biological blood vessels are widely used in clinical practice, which can be divided into two types: allogeneic or heterogeneous. The latter mainly comes from purebred pigs. Biological blood vessels have a natural stent structure, optimal hemodynamics, and do not require anticoagulation. Their preservation techniques and clinical applications have become hot spots in complex cardiac surgery. Among them, allogeneic preservation and application technology is relatively mature, but there is no regulation of brain death law in our country, coupled with the traditional concept of Orientals, it is almost impossible to obt...

AI Technical Summary

Problems solved by technology

The German Haverich research group and the British Ingham research group used various surfactants and proteolytic enzymes to remove the donor cells on the scaffold, and found that there were many problems: (I) low concentration of trypsin could not remove the deep cell components of the arterial wall , a high concentration of trypsin can cause the destruction of the scaffold structure

Its effect is not ideal

[0007] In order to avoid the inadequacies of the above-mentioned chemical method of decellularization, we have studied the physical method of decellularization using ultra-high pressure. high

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