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Extract of acaleph toxin as well as preparation method and usage thereof

A technology for jellyfish toxins and extracts, which is used in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2008-05-21
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But jellyfish toxin extracts with pain-inhibiting and antibacterial activities have not been reported

Method used

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  • Extract of acaleph toxin as well as preparation method and usage thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Refer to Figure 1 and Figure 2. A jellyfish toxin extract is a polypeptide mixture isolated from jellyfish tentacles, and its preparation method is as follows:

[0040] (1) Preparation of freeze-dried powder: use jellyfish tentacles as raw material, divide it into pieces, homogenate the tissue, remove the precipitate by centrifugation, and freeze-dry in vacuum to obtain the freeze-dried powder for later use;

[0041] (2) Sephadex G-10 Desalting Column Desalting: Dissolve the lyophilized powder in purified water and apply it to the G-10 Sephadex Gel Filtration Column, elute with Tris-HCl pH 7.8 buffer, when Measured A 280 Stop elution when it is less than 0.03; put the desalted sample solution collected by the above method into a centrifuge tube, seal it, mark it and put it in a -20°C refrigerator for later use;

[0042] (3) SP Sephadex C-25 ion exchange column chromatography: Biologic Duoflow protein chromatography system was used to conduct gradient elution...

Embodiment 2

[0045] Example 2. In the jellyfish toxin extract described in Example 1, the method for preparing the freeze-dried powder described in step (1) is as follows: cut the jellyfish tentacles into small pieces, add an appropriate amount of phosphate buffer, and use a high-speed tissue masher Homogenate the tissue and collect the homogenate; take out the supernatant by high-speed refrigerated centrifugation; pour the supernatant onto a flat plate and seal it with plastic wrap in a -40°C freezer to freeze, then put it into a vacuum freeze dryer to freeze-dry to become a freeze-dried powder, pack Store in a centrifuge tube at -20°C for later use.

Embodiment 3

[0046] Example 3. In the jellyfish toxin extract described in Example 2, the added phosphate buffer was 0.1M and the pH was 6.0.

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Abstract

The invention relates to a jellyfish toxin extract, which is characterized in that the invention obtains polypeptide mixture by the separation of jellyfish tentacle, the preparation method is as follows: (1) the preparation of freeze-dried powder: the jellyfish tentacle is taken as the raw material and is split into blocks, and the freeze-dried powder is obtained for standby by tissue homogenization, centrifugalization, removal, sedimentation and vacuum freeze-drying; (2) desalination is carried out by sephadex G-10 desalination column; (3) chromatography is carried out by SP sephadex C-25 ion exchange column, so as to collect the separating substances of a fifth peak; (4) the chromatography by SP sephadex C-25 ion exchange column is carried out for the separating substances of the fifth peak, so as to collect the separating substances of a sixth peak and obtain the invention. The invention also discloses a preparation method and the usage of the jellyfish toxin extract. The jellyfish toxin extract of the invention is the polypeptide mixture which is firstly separated from the jellyfish tentacle, and the jellyfish toxin extract has stronger suppression of pain and antibacterial activity, so the invention is conductive to jellyfish for the obtainment of high-value development, in particular to rhopilema and other low-value resources.

Description

technical field [0001] The invention relates to a marine organism extract, in particular to a jellyfish toxin extract; the invention also discloses a preparation method and application of the jellyfish toxin extract. Background technique [0002] Analgesics refer to a class of drugs that can selectively suppress pain sensation and relieve pain. There are two main types of analgesic drugs widely used at present: the first type is a receptor agonist that binds to opioid receptors in the central nervous system, mimics the function of endogenous analgesic substances, activates the anti-pain system in the body, and exerts analgesic effects. The second category is non-steroidal anti-inflammatory analgesics that inhibit the pain of body nerve conduction by inhibiting the prostaglandin synthase in the hypothalamic thermoregulation center. The former often has severe tolerance and drug dependence, while the latter has limited analgesic effect. [0003] Biotoxins are natural active ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61P25/04A61P29/00A61P31/00A61K35/614
Inventor 李联泰高健安贤惠
Owner HUAIHAI INST OF TECH
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