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Strain and method for preparing D-allulose by microbial transformation of D-levulose

A technology of allulose and fructose, which is applied in the field of converting D-fructose to prepare D-psicose, which can solve the problems of low content, unsuitability for large-scale industrial production, and high cost of natural extraction and separation

Active Publication Date: 2008-05-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since D-psicose is a relatively rare monosaccharide, its content in nature is extremely low, the cost of natural extraction and separation is high, and it is not suitable for the requirements of large-scale industrial production. However, the chemical catalytic method has many disadvantages, such as the formation Numerous by-products and chemical contaminants that complicate purification steps

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] SK011 was used for culture and fermentation, and the slant medium was glucose nutrient agar medium. The composition of the seed medium is glucose 3g / L; corn steep liquor 20g / L; urea 10g / L; K 2 HPO 4 .3H 2 O 0.8g / L; KH 2 PO 4 2.5g / L, MgSO 4 .7H 2O 0.9g / L; pH 7.0; Seed medium culture conditions: Use 250mL Erlenmeyer flask to fill 50mL medium, sterilize, cool, and inoculate according to conventional methods, and then culture in a shaker at 30°C and 180r / min for 24h as a seed culture medium. The composition of the fermentation medium is: corn steep liquor 30g / L, urea 2g / L, K 2 HPO 4 .3H 2 O 3g / L, KH 2 PO 4 3g / L, MgSO 4 .7H 2 O 1g / L, pH 7.0, prepared with deionized water; fermentation conditions: use 250mL Erlenmeyer flask to fill 45mL medium, sterilize, cool, and inoculate according to conventional methods, and then culture at 33°C and 180r / min shaker for 12h, then add 1.5g / L of D-tagatose was used as an inducer to continue culturing for 24 hours, and finally...

Embodiment 2

[0035] According to the method of Example 1, SK011 was used to culture and ferment, and the obtained wet bacteria were 0.8g, and 5% cetyltrimethylammonium bromide was added to permeabilize the cells, and the cells were washed with physiological saline after being treated at room temperature for 20 minutes. After 2 times, add 10mL of 10% D-fructose solution into a 50mL Erlenmeyer flask, the initial pH value is 8.0, 25°C, 180r / min shaking transformation for 12 hours, the D-psicose concentration in the reaction solution is measured to be 6g / L .

Embodiment 3

[0037] According to the method of Example 1, SK011 was used to culture and ferment, and 9 g of wet cells were obtained, and 1% toluene was added to permeabilize the cells. After 20 minutes at room temperature, the cells were washed twice with normal saline, and 10 mL of 50% D-fructose solution, initial pH value 10.0, 45°C, 180r / min shaking transformation for 1 hour, the D-psicose concentration in the reaction solution was measured to be 46g / L.

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PUM

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Abstract

The invention discloses a strain for producing D-piscose by using microorganism to transform D-fructose and a preparation method thereof, pertaining to food biotechnology field. The invention relates to a strain of spherical Rhodobacter sphaeroides SK011 screened from bottom mud of a fish-pond with the preservation number, CCTCC NO: M 207185, and the preparation method that the spherical Rhodobacter sphaeroides SK011 is used for transforming the D-fructose and producing the D-piscose through culture and fermentation. The invention adopts the SK011 as the strain and a fermentation culture medium which consists of carbon and nitrogen sources, inducer and inorganic salt; the obtained strain is further treated, so as to obtain cell biocatalyst; then by using the D-fructose as a substrate to do bio-transformation, the D-piscose is prepared. Under optimum conditions during the fermentation culture, fermenting solution or free cells or permeability cells or frozen dry powder or solidified cells thereof are used for transforming the D-fructose for 0.5 to 4.8 hours, and the content of the D-piscose in the transformation solution is 2 to 50g / L.

Description

technical field [0001] A strain and method for preparing D-psicose by transforming D-fructose with microorganisms. The present invention relates to a strain of Rhodobacter sphaeroides SK011 obtained through screening, the preservation number of which is: CCTCC NO: M207185, and its cultivation and fermentation. And it is used in the method of converting D-fructose to prepare D-psicose. It belongs to the field of food biotechnology. Background technique [0002] In recent years, functional food has become the focus of attention of consumers, and it is also the focus of research and development of food practitioners. The increase and younger age of people with diabetes, obesity and cardiovascular disease make functional sweeteners with low calories and more functional nutritional properties become a hot spot of attention. [0003] D-Psicose is a kind of natural ketohexose which is relatively rare in nature, and belongs to a kind of rare sugar. The International Society of Ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/02C12R1/01
Inventor 江波沐万孟张龙涛
Owner JIANGNAN UNIV
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