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Method for producing glucuronic acid and/or glucuronolactone

A technology of glucuronolactone and glucuronic acid, which is applied in the field of preparation of glucuronic acid and/or glucuronolactone, can solve the problems of high cost, high price, and low hydrolysis ability, and achieve Excellent production efficiency and low equipment investment

Inactive Publication Date: 2008-04-30
ENSUIKO SUGAR REFINING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In this method, the yield of the raw material is good, but the required amount of oxidation catalyst is large, the cost is high, and the glucosidic bond of the intermediate raw material trehalose carboxylic acid is stable, so if the relatively violent acid hydrolysis reaction is not carried out, the glucuronic acid will not will dissociate
There are also methods using enzymes such as α-glucuronidase, which are usually more expensive and do not have high hydrolytic power

Method used

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  • Method for producing glucuronic acid and/or glucuronolactone
  • Method for producing glucuronic acid and/or glucuronolactone
  • Method for producing glucuronic acid and/or glucuronolactone

Examples

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Effect test

Embodiment 1

[0066] The present invention is further described in detail through the following examples, but the present invention is not limited thereto.

[0067] (1) Preparation of sucrose carboxylic acid sodium salt

[0068] According to the method described in Example 1 of Japanese Patent No. 3556690, an oxidase-producing bacterium was used to prepare a solution of an equivalent amount of sucrose carboxylate sodium salt (glucurono-fructoside) from 360 kg of sucrose (white sugar). That is, add 30kg sucrose and 250L water to the fermenter, dissolve, then add 50 L of Pseudogluconobacter saccharoketogenes washing bacteria liquid prepared in the same amount of fermenter to the solution prepared above, so that the total amount is 300L. Next, at 32° C., while stirring at 200 rpm, air was blown at a rate of 100 L / min, and the reaction was carried out for 24 hours to obtain the target sodium sucrose carboxylic acid salt. Then remove the bacterium, use again according to whether there is activi...

Embodiment 2

[0079] According to the method for Example 1, an equivalent amount of potassium salt of sucrose carboxylate was prepared from 15 kg of sucrose.

[0080] 100 g of potassium salt of sucrose carboxylate was taken therefrom, 7 g of commercially available baker's yeast (dry yeast) was added thereto, and hydrolysis reaction and assimilation reaction were performed at 37°C.

[0081] As a result, sucrose carboxylic acid was hydrolyzed within 24 hours to obtain a solution containing glucuronic acid and glucuronolactone. Then, the solution was passed through 1L activated carbon column (made by Egret, Japan EnviroChemicals, Ltd.), 1L basic ion exchange resin (Amberlite IRA-96SB, manufactured by Organo Corporation), 3L strongly acidic ion exchange resin (Diaion PK-216, manufactured by Mitsubishi Chemical Co., Ltd.), decolorized and desalted.

[0082] The desalted solution was concentrated to 65% at 40° C., 0.2 g of glucuronic acid seed crystal was added, and cooled naturally. The precip...

Embodiment 3

[0085] According to the method described in Example 1, an equivalent amount of sucrose carboxylic acid sodium salt was prepared from 15kg raw sugar.

[0086] Then also according to the method of Example 1, 15kg of sucrose carboxylic acid sodium salt solution was concentrated to 30%, and then 3% baker's yeast (Oriental Yeast Co., Ltd. preparation, FD-1) was added in terms of solid content, at 37°C The reaction was carried out for 72 hours.

[0087] The reaction solution is also passed through an activated carbon column and a desalting resin column for decolorization and desalination. The resulting desalted solution was concentrated to 58% at 50° C., and 20 g of glucuronolactone seed crystals were added to precipitate crystals. Then, centrifugation was performed to obtain 2 kg of glucuronolactone.

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Abstract

An object of the invention is to provide a method capable of producing D-glucuronic acid and D-glucuronolactone easily and safely in high yield and at low cost. The invention provides a method for producing glucuronic acid and / or glucuronolactone characterized by oxidizing sucrose to form sucrose carboxylic acid (and a salt thereof) (glucuronyl-fructoside, ss-D-fructosyl-(2-1)-a-D-glucuronic acid and a salt thereof), adding yeast or the like thereto to hydrolyze and assimilate the fructose moiety of the sucrose carboxylic acid (and the salt thereof) and collecting produced glucuronic acid and / or glucuronolactone.

Description

technical field [0001] The present invention relates to the preparation method of glucuronic acid and / or glucuronolactone, more specifically, it is characterized in that: make sucrose oxidize, make sucrose carboxylic acid (and its salt) (glucuronic acid base -fructoside, β-D-fructosyl-(2→1)-α-D-glucuronic acid and its salts), and then add microorganisms such as yeast to partially hydrolyze the fructose and assimilate it at the same time, and collect the generated glucose uronic acid and / or glucuronolactone. Background technique [0002] Glucuronic acid and glucuronolactone or derivatives thereof can be widely used in medicines and pharmaceutical raw materials. Various methods for synthesizing these glucuronic acid or glucuronolactone are known. A common method is to obtain glucuronic acid and glucuronolactone by oxidizing glucose derivatives such as glucose derivatives or starch using nitrogen compounds such as nitric acid as raw materials and hydrolyzing them (see Patent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P17/18C12R1/645C12R1/85
CPCC12P19/02C12P17/04C12P7/62
Inventor 滨保健一田所宏基岸野惠理子伊藤哲也藤田孝辉原耕三
Owner ENSUIKO SUGAR REFINING
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