Method for preparing N-carbamyl glutamic acid

Abstract

The invention discloses a method for preparing N-carbamoylglutamic acid. The method comprises the following steps: a. Mixing glutamic acid, potassium cyanate, potassium hydroxide, concentrated hydrochloric acid and urea propionic acid according to 1-1.5: The molar ratio of 1-1.5:1-1.5 is fully mixed to obtain the mixture; b. The mixture obtained in step a is dissolved in 1L of distilled water per 1-1.5mol, and placed at room temperature at 20-25°C for 16-20 hours to obtain Contain the liquid of the mixture described in step a of 1-1.5mol in every unit of liquid; c. acidify each unit of liquid described in step b with 80-100ml of concentrated hydrochloric acid, then stand in 200ml of ureapropionic acid for 2-3 hours After filtering, N-carbamylglutamic acid was recrystallized from water. Compared with other existing methods for producing N-carbamylglutamic acid, the present invention has the following advantages: 1. The method uses cheap glutamic acid as a raw material, which greatly reduces the production cost. 2. The production process is carried out at room temperature, and the process is simple. 3. N-carbamoylglutamic acid recrystallized from water has no toxic and side effects. The invention provides a practical way for really realizing the use of N-carbamyl glutamic acid as arginine substitute.

Description

technical field [0001] The present invention relates to a preparation method of N-carbamylglutamic acid. Background technique [0002] Arginine is an essential amino acid for suckling piglets. The amount of arginine in breast milk and the endogenous synthesis in the intestine of suckling piglets cannot meet their optimal growth needs. Insufficient arginine limits the optimal growth of suckling piglets. a major factor. Piglets have a high demand for arginine, and the arginine content in breast milk is 1.34g / L, which can meet about 40% of the piglets' requirements. The concentration of arginine and its synthetic precursors in the plasma of piglets decreased from 3 to 14 days after birth. One of the reasons was that the concentration of amino acids and proteins in breast milk decreased due to the prolonged lactation period due to the increase of protein deposition required for the growth of pigs. The production of suckling piglets is very important to the whole pig production...

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Problems solved by technology

[0003] Some data show that carbamoyl phosphate is necessary for the synthesis of citrulline from ornithine, and N-acetylglutamic acid is a metabolic allosteric activator of carbamoyl phosphate synthase and P5C synthase (Susumu K., et al. in vitro Effects of adding disulfide or thiol compounds on the activity of arginine activator N-acetylglutamate synthase. Molecular Catalysts B: Enzymes. 2000, 10: 191-197), but in the small intestinal mucosa and Insufficient N-acetylglutamate synthetase in the mitochondria of the liver leads to insufficient synthesis of N-acetylglutamate (Uchiyama, C et al. The subcellular localization and Properties. Biochemistry. 1981, 89:1777~1786.), therefore, N-acetylglutamic acid may play an important role in regulating the synthesis of citrulline and arginine in the small intestine of suckling piglets