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Novel use for issatchenkia orientalis

A technology of ESAB yeast and yeast extract, which is applied in the application field of lignocellulose hydrolyzate fermentation to achieve the effect of reducing costs

Inactive Publication Date: 2008-04-23
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Novel use for issatchenkia orientalis
  • Novel use for issatchenkia orientalis
  • Novel use for issatchenkia orientalis

Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and identification of Y4

[0031] Y4 separation method: collect soil samples from a depth of 1-5 cm below the surface of the soil near a furfural plant, activate the collected soil samples and add them to YPD medium for enrichment culture, and then take 10 mL of the enriched bacteria The solution was connected to the medium containing 90mL hydrolysate (yeast extract 3g / L, KH 2 PO 4 3g / L, malt extract 3g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 .7H 2 O1g / L, tryptone 3g / L, the rest is lignocellulose hydrolysate stock solution, adjust the pH to 5.0-6.0) in a 250mL Erlenmeyer flask, incubate at 80rpm and 30℃ for 48h, then take 10mL of this culture solution and connect it to 90mL In the same fresh medium, continue to incubate for 48 hours at 80 rpm and 30°C. Repeat this procedure. After 4-5 transfers, take 10 uL of the culture solution after a certain gradient dilution and spread it on the solid plate of the same medium. After 48 hours of culture at 30°C, a single colo...

Embodiment 2

[0049] Example 2 Ethanol fermentation of Y4

[0050] The Y4 strain was inoculated to 3g / L, KH containing yeast extract 2 PO 4 3g / L, malt extract 3g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 .7H 2 O 0.5g / L, tryptone 3g / L, hydrolysate stock solution, pH5.0 culture medium, 30℃, 80rpm culture, the glucose is used up within 24h, and during the fermentation process, the xylose is also gradually reduced, after 72 After hours, xylose was completely consumed. The highest yield of ethanol reached 96.1% of the theoretical value (theoretical yield was 0.51 g / g).

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Abstract

The present invention provides the application of Issatchenkia orientalis in fermenting lignocellulose hydrolysate. Issatchenkia orientalis and its domesticated strain is applied in fermenting lignocellulose hydrolysate to degrade the fermentation inhibitor fast, to metabolize glucose for producing ethanol and to lower the cost of producing ethanol with lignocellulose. The present invention lays foundation of large scale industrial production, and provides also one Issatchenkia orientalis strain Y4 with high fermentation efficiency.

Description

Technical field [0001] The invention relates to a new use of Isa orientalis, in particular to the application in the fermentation of lignocellulosic hydrolysates. Background technique [0002] The production of ethanol from non-food material-lignocellulosic raw materials is one of the important ways to ensure national energy security, protect the environment, and make my country's economy and society sustainable. The composition of lignocellulose hydrolysate: glucose + xylose mixed sugar concentration of 6%, acetic acid 10g / L, furfural 2g / L, hydroxymethyl furfural 1g / L, and contains phenolic compounds and aromatic hydrocarbon compounds. The high-efficiency and rapid hydrolysis of lignocellulose and the high-efficiency ethanol fermentation of hydrolysate are two technical bottlenecks for the industrial production of fuel ethanol at home and abroad. In addition to mixed sugars, lignocellulose hydrolysates also contain fermentation inhibitors such as furfural, hydroxymethyl furfural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/10
CPCY02E50/16Y02E50/10
Inventor 杨秀山
Owner CAPITAL NORMAL UNIVERSITY
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