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Application of fusion protein GST-PTD-SOD in radioresistance

A technology of GST-PTD-SOD and fusion protein, which is applied in the field of fusion protein, can solve problems such as inability to effectively eliminate cell damage, achieve the effects of repairing radiation-damaged cells, protecting cells from radiation damage, and increasing differentiation

Inactive Publication Date: 2008-03-19
FUZHOU UNIV
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  • Claims
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Problems solved by technology

However, after radiation, a large number of free radicals are produced inside and outside the cells. Simply removing the free radicals outside the cells by administering drugs from the outside of the cells cannot effectively eliminate the damage caused by radiation to the cells. Therefore, SOD products are not widely used as common drugs for radiation damage. in treatment

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  • Application of fusion protein GST-PTD-SOD in radioresistance

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Embodiment 1

[0015] Example 1: Cloning hCu from human hepatocytes by RT-PCR, Zn SOD cDNA, then using primer 1 containing the coding gene of TAT-PTD at the 5' end and a primer complementary to the 3' end of hCu, Zn SOD cDNA 2 Do PCR amplification to obtain hCu, ZnSOD and PTD fusion genes. After the amplified product was recovered by enzyme digestion and electrophoresis, it was inserted into the multiple cloning site of the plasmid pGEX 2T, the expression plasmid pGEX-PTD-SOD was constructed, transformed into Escherichia coli BL21(DE3), and positive clones were screened for identification by enzyme digestion.

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Abstract

The invention relates to the application of fusion protein GST-PTD-SOD under the radiative condition, wherein, the GST-PTD-SOD is the fusion protein GST-PTD-SOD in which glutathione s-transferase (GST) and TAT protein transduction domain are connected with superoxide dismutase, and a substance containing the GST-PTD-SOD is applied in radiation resisting. The fusion protein GST-PTD-SOD can increase the activity of the SOD in cells; because the transmembrane efficiencies of the fusion protein GST-PTD-SOD are different in various cells and the demand degrees of various cells are different in terms of free radials, the fusion protein GST-PTD-SOD has different protection and reparation effects on various cells, and relatively speaking, the fusion protein GST-PTD-SOD has a better protection and reparation effect on some normal cells than on cancer cells. The fusion protein GST-PTD-SOD can be used as the effective component of anti-radiation injury drugs and has the efficacies of increasing the anti-radiation ability of cells, increasing the differences of the radiation sensitivity of various cells and repairing radiation-injured cells.

Description

Technical field: [0001] The invention relates to the field of fusion proteins, especially the fusion protein of glutathione-S-transferase (GST) and TAT protein transduction domain (Protein Transduction Domain, PTD) linked to superoxide dismutase (Superoxide Dismutase, SOD) Application of GST-PTD-SOD in anti-radiation. Background technique [0002] With the development of radiation technology, people have more and more opportunities to be exposed to radiation sources in daily life. There are many types of radiation sources, including X-rays, gamma rays, radionuclides used in medical treatment, short-wave ultraviolet rays commonly used in laboratories, and medium-wave and short-wave ultraviolet rays that exist in large amounts in sunlight. These radiation sources have a great effect on the benefit of human beings, but the radiation damage they produce cannot be ignored. [0003] The mechanism of radiation damage is complex, but experiments have shown that the damage of oxyge...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K38/16A61K8/64A61Q17/04
Inventor 刘树滔饶平凡
Owner FUZHOU UNIV
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