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Rotifer Brachionus plicatilis microkernel preparation technology

A technology of Brachionus rugosa and folds, applied in the technical field of micronucleus preparation of Brachionus rugosa

Inactive Publication Date: 2008-02-27
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as the environmental detection of water pollutants is concerned, it is an ideal indicator to make full use of plankton in the water body, especially zooplankton to detect the degree of pollution. The use of nuclear technology as a detection indicator is still in a blank state

Method used

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  • Rotifer Brachionus plicatilis microkernel preparation technology

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Embodiment Construction

[0005] The present invention can be realized through following several operation steps:

[0006] 1. Collection and identification of rotifers: Take water samples from the water samples containing Brachionus rugosa in the wild, filter through a filter net, concentrate the liquid containing rotifers, absorb a single rotifer with a siphon, and place it on a glass slide. Identify the species of rotifers under a dissecting mirror (4×10=40 times mirror), and pick out Brachionus rugosa.

[0007] 2. Expanded cultivation of rotifers: Select about 1,000 Brachionus rugosa rotifers from the water samples collected in the field above and put them into 6-well cell culture plates for expanded cultivation. During the cultivation process, freshwater chlorella was selected as the bait of Brachionus plicata: when freshwater chlorella was fed, the ratio of chlorella to rotifer culture solution was 1:50.

[0008] 3. Heavy metal cadmium ion attack culture: After the above rotifers are concentrated...

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Abstract

The invention is a preparation technology of Brachionus plicatilis microkernel. The water pollution (such as heavy metal cadmium) is dangerous to the Brachionus plicatilis. In cell, the cell nuclear fission of Brachionus plicatilis is influenced and the microkernel can be produced. The microkernel number can appraise the toxicity degree of pollution. The preparation method includes the rotifer toxicity, tabletting, coloration and observation. The figure in abstract shows the size and form of the Brachionus plicatilis microkernel.

Description

Technical field: [0001] The invention relates to a preparation method of rotifer micronucleus and its application as a pollutant toxicity index, and is a technique for detecting the toxicity of environmental pollutants by applying cytotoxicology. Background technique: [0002] Among chemical substances, there are many that can cause chromosomal abnormalities. Chromosomal abnormalities induced by chemical substances are manifested as micronuclei due to chromosome loss or fragment formation at the nuclear level. Detection of the probability of micronuclei can be used as an indicator to measure the toxicity of chemical substances . At present, domestic and foreign studies on the response of organisms to environmental pollutants from the perspective of cytotoxicology focus on the following aspects; one is to use the response of plant root tip cells to heavy metal toxicity during mitosis, such as broad bean root tip, onion Plant roots such as root tips and garlic root tips were ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N1/28C12Q1/02
CPCY02A20/20
Inventor 高焕陈建华徐静李晓英赵艳景阎斌伦
Owner HUAIHAI INST OF TECH
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