Porphyra haitanensis germ plasma identification fingerprint construction method and its uses

Abstract

The utility model relates to a reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis, belonging to fingerprint chromatogram of porphyra haitaensis, in particular to reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis. The utility model provides a rapid accurate reconstruction method for identifying fingerprint chromatogram of porphyra haitaensis and the application for realizing the porphyra haitaensis identification automation, which is characterized in that porphyra haitaensis specimens are collected; genome DNA of various specimens is refined and purified; the PCR is enlarged, the refined genome DNA is utilized as a template, SRAP molecule marking analysis is performed by the optimized experimental system; the representative SRAP multi-state bars are selected for reconstructing the DNA fingerprint chromatogram of various specimens; the reconstructed DNA fingerprint chromatogram is converted to the identifiable digital fingerprint by computer, and then is inputted to the fingerprint chromatogram identification software, thereby the digital fingerprint database of porphyra haitaensis is established. The utility model is used for follow-up porphyra haitaensis automation identification.

Description

technical field [0001] The invention relates to a fingerprint of Porphyra alba, in particular to a method for constructing a fingerprint of Porphyra germplasm identification and its application. Background technique [0002] Porphyra haitanensis is a unique species of warm temperate zone in my country. It is an important economic red algae artificially cultured in coastal areas of Fujian, Zhejiang and Guangdong. Its output accounts for about 80% of the total national laver production. In the past, the classification of Porphyra mainly relied on morphological methods. However, because most morphological characters are greatly affected by environmental factors, the descriptive characteristics are limited, and it is difficult to meet the requirements of Porphyra germplasm identification and improved breeding. The emergence of molecular marker technology has brought an opportunity to solve this problem. It directly marks and detects the genetic variation of the genome at the DNA...

AI Technical Summary

Problems solved by technology

[0003] SRAP—Related Sequence Amplified Polymorphism Marker is a new type of molecular marker technology with high polymorphism, medium yield, good repeatability, simple operation, uniform distribution in the genome, and easier detection of amplified The target fragment is sequenced, and the primers are versatile, and the forward primer can be combined with the reverse primer in pairs, and multiple primer pairs can be assembled with a small number of primers, thereby improving the efficiency of primer use and reducing the cost of primer synthesis. With many advantages, it has been widely used in plant genetic diversity analysis, fingerprint map construction, germplasm identification and genetic linkage map drawing (see literature: Li G, Quiros CF. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCRreaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455-461), but the application in seaweed has not been reported so far

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