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Application of dihydroartemisinin in reinforcing chemotherapy medicine antitumor curative effect

A technology of dihydroartemisinin and chemotherapeutic drugs, which can be used in antitumor drugs, medical preparations containing active ingredients, drug delivery, etc., and can solve problems such as limiting long-term application

Inactive Publication Date: 2008-02-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemotherapy or radiotherapy can cause most tumors to regress, their long-term application is limited due to serious adverse reactions of chemotherapy drugs or radiotherapy

Method used

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  • Application of dihydroartemisinin in reinforcing chemotherapy medicine antitumor curative effect
  • Application of dihydroartemisinin in reinforcing chemotherapy medicine antitumor curative effect
  • Application of dihydroartemisinin in reinforcing chemotherapy medicine antitumor curative effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Dihydroartemisinin enhances the therapeutic effect of cyclophosphamide on the growth and metastasis of Lewis lung cancer

[0028] Test materials: Dihydroartemisinin tablets (Zhejiang Huali Nanhu Pharmaceutical Co., Ltd.), LLC (LewisLung Carcinoma) cell line (provided by Shanghai Institute of Cell Biology, Chinese Academy of Sciences), 100 C57BL / 6 mice, male, weighing 20 ±2g, 6-8 weeks old (provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences), immunohistochemical Elivision TM plus broad-spectrum kit (KIT-9901 / 9902 / 9903) (Fuzhou Maixin Biotechnology Co., Ltd.), mouse anti-human VEGF monoclonal antibody (Santa Cruz Company).

[0029] method:

[0030] (1) Establishment of Lewis lung cancer growth and metastasis model and drug regimen

[0031] Mouse LLC cells were cultured in DMEM medium containing 10% calf serum at 37°C, 5% CO 2 Incubate in the cell culture incubator and subculture after 70-90% of the area is covered. Take the cells ...

Embodiment 2

[0071] Example 2: Dihydroartemisinin induces apoptosis of Lewis lung cancer (LLC) cells in vitro and down-regulates expression of VEGF receptor KDR / flk-1

[0072] Dihydroartemisinin: a gift from Guangxi Guilin Pharmaceutical Factory

[0073] method:

[0074] (1) MTT method to detect cell proliferation

[0075] 5×10 4 ml -1 LLC cells were seeded in a 96-well plate, and DMSO was used as a solvent control. After being treated with different concentrations of dihydroartemisinin for 48 hours, the MTT method was used to detect the absorbance at a wavelength of 570 nm (A 570nm ) value, calculate cell proliferation rate, cell proliferation rate (%)=experimental group A 570nm / solvent control group A 570nm ×100%. Using IC 50 Calculation software to calculate the IC of dihydroartemisinin in inhibiting the proliferation of LLC cells 50 Values ​​were calculated with 95% confidence intervals using SPSS10.0 statistical software.

[0076] (2) Morphological detection of apoptosis

...

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Abstract

The present invention provides an application of dihydroartemisinin in the preparation of the drugs to enhance the anti-tumor efficacy of the chemotherapeutic drugs. The pharmaceutical preparation contains preparation allowable pharmaceutical excipients or carriers. The form of the preparation is solid preparation. The pharmaceutical preparation provided by the present invention can enhance the anti-tumor efficacy of the chemotherapeutic drugs and can be used in the adjuvant therapy during the tumor chemotherapy process. The present invention takes the theory that the angiogenesis inhibitor can change the micro-environment of tumor as the background, studies and clarifies the function and the mechanism that the effective monomer in the traditional Chinese medicine can enhance the anti-tumor efficacy of the chemotherapy drugs, provides a pharmacological basis for the development of the new usages of the artemisinin drugs and provides an important basis for the development of the new clinical values of the angiogenesis inhibitor, so the present invention is a new research direction for the development of the theory of traditional Chinese medicine.

Description

technical field [0001] The invention belongs to the medicinal use, and relates to the medicinal use of dihydroartemisinin in enhancing the antitumor curative effect of chemotherapeutic drugs. Background technique [0002] Malignant tumors are one of the most serious diseases threatening human health, and the main reason for their treatment failure is the resistance of tumor cells to chemotherapeutic drugs. In addition to the instinctive mutation of cancer cells, another important reason for the resistance of malignant tumors to chemotherapy drugs is the abnormal structure and function of the tumor tissue microenvironment. The formation mechanism of this abnormal microenvironment has not been fully elucidated, and it is generally believed that it is related to the severe defects in the structure and function of neovascularization in tumor tissue, the increase of interstitial fluid pressure in tissue, hypoxic environment and acidosis and other factors. In recent years, angiog...

Claims

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Application Information

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IPC IPC(8): A61K31/357A61K9/00A61P35/00
Inventor 周慧君李傲王增
Owner ZHEJIANG UNIV
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