Method for producing high temperature stable slow-slaking amidon and uses thereof
A slow-digesting starch, high-temperature stable technology, applied in the fields of medicine, feed, food, and non-chemically modified starch, to achieve high social and economic benefits, easy automatic control, and simple procedures
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Embodiment 1
[0029] The ordinary corn starch milk with a concentration of 5% is gelatinized at a temperature of 50°C for 60 minutes. After the starch paste is cooled, pullulanase, α-amylase and phosphate buffer are added and mixed uniformly. The starch paste is 1kg, and the pullulan is added. Blue enzyme 0.005kg, α-amylase 0.001kg, buffer 1L. The reaction was continuously stirred at 40°C and pH 3.5 for 1 hour. Enzyme inactivation treatment, the enzymatic hydrolysate was stored at 1° C., recrystallized for 5 hours, and then dried. Then add water to adjust to 35% moisture content, the temperature is 50°C, and the temperature is 50°C. The hot liquid airtight heat preservation treatment is 0.01h, and then the high temperature stable slow digestion starch with a content of 58% is obtained after drying and crushing.
Embodiment 2
[0031] The potato starch milk with a concentration of 20% is gelatinized at a temperature of 70°C for 30 minutes. After cooling the starch paste, add pullulanase, α-amylase and phosphate buffer and mix it evenly. The starch paste is 1kg, and pullulan is added. Enzyme 0.03kg, α-amylase 0.01kg, buffer 2L. The reaction was continuously stirred at 70°C and pH 5 for 10 hours. Enzyme inactivation treatment, the enzymatic hydrolysate was stored at 10°C, recrystallized for 10 hours, and then dried. Then add water to adjust to 45% moisture content, a temperature of 40°C and a thermal liquid airtight heat preservation treatment for 6 hours, and then undergo drying and crushing treatments to obtain a high-temperature stable slow digestion starch with a content of 83%.
Embodiment 3
[0033] The mung bean starch milk with a concentration of 40% is gelatinized at a temperature of 150°C for 5 minutes. After cooling the starch paste, add pullulanase, α-amylase and phosphate buffer to mix evenly. The starch paste is 1kg, and pullulan is added. Enzyme 0.Skg, α-amylase 0.3kg, buffer 3L. The reaction was continuously stirred at 90°C and pH 6 for 24 hours. After enzymatic inactivation treatment, the enzymatic hydrolysate was stored at 20° C., recrystallized for 24 hours and then dried. Then add water to adjust to 60% moisture content, 30°C hot liquid airtight heat preservation treatment for 20 hours, and then dry and pulverize to obtain high temperature stable slow digestion starch with a content of 78%.
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