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Method for making new, branch and flow H9 sub-type tri-combined inactived vaccine

A technology of inactivated vaccines and inactivated methods, which is applied in the field of poultry biopharmaceuticals, can solve the problems of increasing production costs, affecting production performance, increasing labor intensity, etc., and achieves the effects of saving time, saving the number of uses, and reducing operation steps

Inactive Publication Date: 2008-01-30
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the long-term strategic concept of disease prevention and control, as far as traditional vaccines are concerned, the weight of inactivated vaccines will be gradually increased, and the use of live vaccines, especially moderately virulent live vaccines, will be reduced. There are more and more types and times of animal vaccinations. Repeated immunizations with multiple injections, large doses, and multiple varieties of inactivated vaccines will cause stress reactions and allergic reactions in livestock and poultry, affecting production performance.
At the same time, multiple vaccinations will increase labor intensity and increase production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0019] Preparation of virus seeds for production NDVLaSota strain, IBVM41 strain and AIVHL strain virus seeds were diluted appropriately, respectively inoculated with SPF chicken embryos, incubated, and harvested; preparation of virus liquid for seedling production: NDVLaSota strain, IBV M41 strain and AIVHL strain liquid respectively Appropriately dilute, inoculate susceptible chicken embryos respectively, and incubate; collect live virus seeds: respectively concentrate and inactivate, take 11% of NDVLaSota strain liquid, 11% of IBVM41 strain liquid, and 10% of AIVHL strain liquid for seedling preparation. Wen-80 is used to make the water phase, and white oil, Siben-80, and aluminum stearate are used to make the oil phase. The ratio of the water phase to the oil phase is 1:3, and they are emulsified together, divided, labeled, and boxed. storage.

specific Embodiment approach 3

[0020] Preparation of virus seeds for production NDVLaSota strain, IBVM41 strain and AIVHL virus seeds were diluted appropriately respectively, respectively inoculated with SPF chicken embryos, incubated, and harvested; preparation of virus liquid for seedlings NDVLaSota strain, IBVM41 strain and AIVHL virus strains were appropriately diluted respectively, Inoculate susceptible chicken embryos separately, incubate and collect live virus seeds. Concentrate and inactivate respectively. Take 12% of NDV LaSota strain liquid, 12% of IBVM411 strain liquid and 11% of AIVHL strain liquid and add Tween-80 to make seedlings Water phase. The oil phase is made of white oil, Siben-80, and aluminum stearate. The ratio of the water phase to the oil phase is 1:4 and emulsified together. Packing, labeling, packing, and storage.

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Abstract

The present invention relates to a preparation method of a triple inactivated vaccine for the prevention of H9 subtype newcastle disease, infectious bronchitis and avian influenza, which is characterized in that: the content of the using NDVLaSota stain virus liquid is 10 to 22 percent, the content of IBVM41 stain virus liquid is also 10 to 22 percent and the content of the AIVHL stain virus liquid is 8 to 15 percent; the present invention uses the concentration and inactivation methods; the white oil adjuvant and the immune auxiliaries of Tween, Span and aluminum stearate are added to prepare the triple inactivated vaccine; the present invention realizes the multiple vaccine prevention and achieves the purpose of prevention a plurality of diseases by one needle, which can be used for greatly developing the husbandry, so as to benefit the mankind and improve the living standard of the people.

Description

Technical field: [0001] The invention relates to the technical field of poultry biopharmaceuticals, and relates to a method for preparing a triple inactivated vaccine of new, tributary and stream H9 subtypes Background technique: [0002] Avian influenza first appeared in Italy in 1878. It is a viral infectious disease caused by type A orthomyxovirus. According to the antigenicity of influenza virus hemagglutinin HA and neuraminidase NA, it can be divided into many subtypes, such as H5N1, H9N2. The disease mainly affects the respiratory, digestive and genitourinary systems of poultry. The main clinical manifestations are respiratory symptoms, egg production rate drop and death in varying degrees. It is a class A infectious disease, and its transmission route is mainly through horizontal transmission, and chickens of different ages can be infected in any season; chicken Newcastle disease, commonly known as chicken plague, is a highly contagious infectious disease caused by c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61K39/145A61K39/215A61K39/295A61P31/12
Inventor 孙进忠张许科乔荣岑舒德广闵亚杰孟照国李根龙
Owner PU LIKE BIO ENG
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