Physic nut polyploid inducement method under the isolated culture condition

A technology for in vitro culture and jatropha, which is applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problem of low induction frequency and achieve the effect of improving stress resistance and promoting the development of renewable energy of jatropha

Inactive Publication Date: 2008-01-30
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

Because the induction method of the whole plant level is simple and easy to grasp, the polyploidy induction method of flowers is mainly used at present, but the induction frequency is low, and sometimes some plants are chimeras
But so far there is no report on polyploid breeding of renewable energy - Jatropha curcas

Method used

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Embodiment Construction

[0019] The present invention is further illustrated by the following examples. It should be noted that: the following examples are illustrative, not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0020] Colchicine induction method Jatropha curcas polyploid induction method steps are as follows under in vitro culture conditions:

[0021] 1. Jatropha seeds were sterilized by 75% alcohol for 30s, and then 0.1% HgCl 2 Disinfect for 6Min, rinse with sterile water for 4 times, peel off the outer testa with tweezers in an ultra-clean bench, inoculate the seeds on the medium of 1 / 2MS+10g / L sucrose, and wait for germination for later use.

[0022] 2. get the blade and the petiole of the Jatropha aseptic seedling of cultivating 5-7d, cut into the segment of 2cm with scissors in ultra-clean bench as explant, place respectively in different colchicine ratio (0.05-0.2%) Culture in differentiation medium (MS+2.0mg / L6-BA+0.1mg / ...

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Abstract

The invention relates to a jatropha polyploidy induction method in the condition of isolated culture and is divided into such steps as jatropha aseptic seedling cultivation, polyploidy induction, polyploidy regenerated bud cultivation, selecting stable variants and rooting induction of variants in rooting cultivation medium containing active carbon, seedling adaptation in matrix and transport of seedling to land after survival. By adopting colchicines to induce the polyploidy of chromosome under isolated condition, the invention can easily control test conditions and repeat test result, thereby improving working efficiency, obtaining a plurality of plants with polyploidy chromosomes and reducing or avoiding heteroploidy cell chimerism. The invention also has actual significance in improving stress resistance of jatropha and can promote the development of renewable resources of jatropha.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for inducing polyploidy of Jatropha curcas under in vitro culture conditions. Background technique [0002] Most plants in nature are diploid (2n=2x), that is, the nuclei of their somatic cells (2n) contain 2 identical chromosome sets (x). However, some species can form new species containing multiple chromosome sets through natural or artificial doubling of chromosomes, which we call polyploidy. Common polyploids are three, four, five, six and octaploid, etc., and 2n is equal to 3x, 4x, 5x, 6x and 8x respectively. Polyploidy is one of the most common characteristics of higher organisms, which is manifested as the number of chromosomes of closely related organisms in genetics. Polyploids are widely distributed in nature. According to statistics, about 1 / 2 of the species in the plant kingdom are polyploids, while polyploids in Poaceae account for almost 3 / 4, and ...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00C12N5/04A01G31/00
Inventor 杨静慧刘艳军张伟玉杨恩芹
Owner TIANJIN AGRICULTURE COLLEGE
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