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Preparation of nano fibre carrier for loading and slow releasing pharmaceutical and biologically active factor

A bioactive factor, nanofiber technology, used in fiber processing, drug delivery, spinning solution preparation, etc., can solve problems such as insufficient water volatility, easy protein inactivation, and failure.

Inactive Publication Date: 2008-01-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the volatility of water is insufficient, so it is generally not used as a solvent for electrospinning, and proteins are easily inactivated in organic solvents, and many drugs will react with organic solvents, thus losing efficacy
Simply dissolving proteins or drugs in organic solvents for electrospinning cannot maintain their application value, so it is necessary to avoid contact between proteins or drugs and organic solvents during the spinning process

Method used

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  • Preparation of nano fibre carrier for loading and slow releasing pharmaceutical and biologically active factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 0.3g gelatin, 0.3gBSA and 0.01mgNGF respectively with an electronic balance, and dissolve them in 10ml ultrapure water under aseptic conditions at the same time, stir and oscillate to dissolve completely to obtain the inner layer solution; electronic balance weighs 0.5g P( LLA-CL)[50:50], dissolved in 10ml of trifluoroethanol, stirred and oscillated until completely dissolved, to obtain the outer layer solution; coaxial electrospinning, set the flow rate of the inner layer solution to 0.2ml / h, and spin The diameter of the head is 0.9mm, the flow rate of the outer layer solution is 0.5ml / h, and the diameter of the spinneret is 1.2mm; 15kv is applied, and the distance between the nozzle and the receiving plate is 8cm, and the spinning is carried out; a "shell-core" structure nanometer with a diameter of about 200mm is obtained. The fiber, wherein the core layer is about 80 nanometers.

Embodiment 2

[0028] Take by weighing 0.3g gelatin, 0.3gBSA and 0.01mgVEGF respectively with an electronic balance, and dissolve it in 10ml ultrapure water simultaneously under aseptic conditions, stir and oscillate to dissolve completely to obtain the inner layer solution; electronic balance weighs 0.5g P( LLA-CL)[50:50], dissolved in 10ml of trifluoroethanol, stirred and oscillated until completely dissolved, to obtain the outer layer solution; coaxial electrospinning, set the flow rate of the inner layer solution to 0.2ml / h, and spin The diameter of the head is 0.9mm, the flow rate of the outer layer solution is 0.5ml / h, and the diameter of the spinneret is 1.2mm; 15kv is applied, and the distance between the nozzle and the receiving plate is 8cm, and the spinning is carried out; a "shell-core" structure nanometer with a diameter of about 200mm is obtained. The fiber, wherein the core layer is about 80 nanometers.

Embodiment 3

[0030] Weigh 0.3g gelatin, 0.3gBSA and 0.1mg heparin with an electronic balance, and dissolve them in 10ml ultrapure water at the same time under sterile conditions, stir and oscillate to dissolve completely to obtain the inner layer solution; weigh 0.5g with an electronic balance P(LLA-CL)[50:50], dissolved in 10ml of trifluoroethanol, stirred and oscillated until completely dissolved to obtain the outer layer solution; coaxial electrospinning, set the flow rate of the inner layer solution to 0.3ml / h, The diameter of the spinneret is 0.9mm, the flow rate of the outer layer solution is 0.7ml / h, and the diameter of the spinneret is 1.2mm; 20kv is applied, and the distance between the nozzle and the receiving plate is 8cm, and the spinning is carried out; a "shell-core" with a diameter of about 220nm is obtained Structural nanofibers, wherein the core layer is about 100 nanometers.

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Abstract

The invention relates to nanometer fiber carrier manufacturing method used to load and release medicine and biological active factors. It includes the following steps: dissolving natural polymer, medicine, or biological active material in ultra pure water to make interface layer solution; dissolving synthetic polymer in volatile organic solvent to make shell solution; setting the distance between spinneret and received board, adjusting feeding speed, coaxial electrostatic spinning to obtain nanometer fiber carrier. The method is simple. In addition, the shell-core nanometer fiber not only can increase its mechanical properties, but also can keep the activity of the loaded medicine and biological active factor which can be used in tracheal tissue engineering support.

Description

technical field [0001] The invention belongs to the preparation of a nanofiber carrier, in particular to the preparation of a nanofiber carrier that loads and slowly releases medicine and bioactive factors. Background technique [0002] In 1934, American Formhals first invented the electrospinning technology, which has since been widely used to prepare tissue engineering scaffolds. After the efforts of later researchers, the coaxial electrospinning technology was invented on the basis of electrospinning, which provided a new method for the sustained release of drugs. However, water is not volatile enough, so it is generally not used as a solvent for electrospinning, and proteins are easily inactivated in organic solvents, and many drugs will react with organic solvents, thus becoming invalid. Simply dissolving proteins or drugs in organic solvents for electrospinning cannot maintain their application value, so it is necessary to avoid contact between proteins or drugs and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01D1/02D01D5/34A61K47/34A61K9/00A61K38/18
Inventor 莫秀梅李晓强何创龙黄晨
Owner DONGHUA UNIV
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