Method for extracting fructus momordicae tetranuclear triterpene

Abstract

A method for extracting fructus momordicae tetranuclear-triterpene is carried out by leaching flavine from fresh fructus momordicae skin by warm water, heating gelatinous protein in fruit cell, solidifying, crushing, leaching by 60-90 deg. C, de-bonding by pectase, filtering, concentrating and drying to obtain final product. Crude products containing tetranuclear-triterpene 43% 300-350g are extracted from fresh fruits 10000g. It's pure, safe, efficient, simple and cheap. It has no environmental pollution. The pumace can be made into feed.

Description

Technical field: [0001] The invention relates to a method for extracting effective components from fresh Luo Han Guo, in particular to a method for extracting tetracyclic triterpenes from Luo Han Guo. Background technique: [0002] The fruit of Luo Han Guo contains triterpene glycosides and other substances with high economic value and medicinal value. At present, there are many methods for extracting mogrosides from Luo Han Guo: the extraction method disclosed in Chinese patent 87101850 is to crush fresh Luo Han Guo, beat, warm water Extraction, and then use synthetic resin DA201 acrylic type adsorption separation, 50% ethanol to dissolve, D-211 acrylic type anion resin to decolorize, desalt, remove impurities, recover ethanol by distillation, concentrate under reduced pressure with a vacuum pump, and freeze-dry; Chinese patent 200510071877.0 The disclosed extraction method is: fresh Luo Han Guo pulverization→ethanol heating and reflux extraction→macroporous resin separatio...

AI Technical Summary

Problems solved by technology

[0002] The fruit of Luo Han Guo contains triterpene glycosides and other substances with high economic value and medicinal value. At present, there are many methods for extracting mogrosides from Luo Han Guo: the extraction method disclosed in Chinese patent 87101850 is to crush fresh Luo Han Guo, beat, warm water Extraction, and then use synthetic resin DA201 acrylic type adsorption separation, 50% ethanol to dissolve, D-211 acrylic type anion resin to decolorize, desalt, remove impurities, recover ethanol by distillation, concentrate under reduced pressure with a vacuum pump, and freeze-dry; Chinese patent 200510071877.0 The disclosed extraction method is: fresh Luo Han Guo pulverization→ethanol heating and reflux extraction→macroporous resin separation, deionized water and ethanol were eluted to colorless, and then the eluate was concentrated under reduced pressure into a paste, and then normal phase silica gel Column separation, adding chloroform, methanol, and water as eluents, recovering the eluent and concentrating it under reduced pressure to obtain mogroside. These extraction processes: 1. The process is complicated; 2. The recovery rate is low; 3. Attached to the adsorption column The material rich in protein is viscous and abnormal, and if it is not handled in time, it is very easy to rot and stink; The method for extracting triterpene sweet glycosides from Luo Han Guo, the process is to wash the fruit, crush, add water and extract four times by countercurrent method, boil for 30 minutes each time, cool, add pectinase (to hydrolyze pectin) , filtering, sterilizing, and concentrating. This method dissolves the yellow pigment in the pericarp and solidifies the protein in the fruit cells in the fruit juice without leaching the yellow pigment in advance. The product tastes bad and has the green smell of fresh Luo Han Guo And the color is not good-looking, and the application and development of other sweet foods are limited