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Excitant of dopamine transport protein and usage

A technology of transporter and dopamine, applied in the field of biomedicine, can solve the problem that patients cannot get effective treatment and so on

Inactive Publication Date: 2007-12-26
上海国联干细胞技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some progress has been made in the treatment of psychiatric and neurological diseases recently, many patients cannot be effectively treated, which is partly because the drugs are ineffective for them or the drugs have serious side effects such as anti-parasympathetic effects, cardiotoxicity, weight loss, etc. (Hao et al. Edited by Wei. Psychiatry, Fourth Edition, Beijing: People's Health Publishing House, 2001; Kapur, Shitij et al Current Opinion in Psychiatry.17(2):115-121, March 2004.)

Method used

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  • Excitant of dopamine transport protein and usage
  • Excitant of dopamine transport protein and usage
  • Excitant of dopamine transport protein and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0094] Screening and detection of embodiment 1DAT agonist

[0095] 1. Establishment of DAT agonist in vitro screening model

[0096] The activity screening cell lines targeting dopamine transporter (DAT) and γ-aminobutyric acid transporter (GAT-1) were established. The full-length cDNA coding sequence of rat DAT and GAT-1 (the GenBank accession number of DAT is GI310097; the GenBank accession number of GAT-1 is GI204221) was cloned into the polynucleotide of pCDNA3 vector (Invitrogen, USA) by conventional molecular biology methods. In the cloning site, Chinese hamster ovary cells (CHO) were transfected by electroporation, and cultured in 1640 medium containing G418 after 48 hours. After 10 days, all the cells in the control group died, while many cell clones formed in the experimental group. After the clones were picked and cultured for one week, when the cells covered the bottom of the well, the medium was sucked off, and digested with trypsin as above. Cells in each well ...

Embodiment 2

[0115] Example 2. Isolation and composition preparation of luteolin (SZ92)

[0116] 1. Separation and purification of active ingredients of Chinese herbal medicine

[0117] A, the preparation method of water extract

[0118] Select 50-100g of perilla (SZ), a part of Chinese herbal medicine that has a therapeutic effect on mental and nervous system diseases, and decoct in water until boiling. After 40 minutes, filter the decoction, and make it through a freeze dryer (LyoPro3000, Jouan Company, Denmark). Dry powder, save samples for in vitro experiments.

[0119] B. Separation, purification and activity tracking of active ingredients in SZ of Chinese herbal medicine

[0120] Take 4 kg of Chinese herbal decoction pieces SZ, add 5 L of 95% industrial alcohol to extract 4 times at room temperature, each time for 3 days. The combined filtrates were concentrated under reduced pressure. Gained medicinal extract 213g, dissolves with 500ml water. Extracted three times with 500ml of...

Embodiment 4

[0132] Embodiment 4. Luteolin treats morphine addiction in rats

[0133] 1. Drug administration in addiction modeling

[0134] Take 30 clean grade Sprague-Dawley rats, weighing 200±10g, half male and half male. Rats were raised at room temperature of 22-24° C., humidity of 50-70%, normal pressure environment, natural day and night, given basic Chow feed and free access to water (sterilized purified water).

[0135] One week later, the rats were randomly divided into 3 groups: normal saline group, empty liposome group and luteolin (SZ92) liposome-embedded group. Intraperitoneal injection of morphine hydrochloride, 6 times on the first day (8:00, 9:30, 12:00, 15:00, 19:00, 22:00), the doses of morphine were 2, 4, 6, 8, 8, 8mg· kg -1 , twice the next day (8:00, 11:00), the dose of morphine is 8mg·kg -1 3 hours after the last injection of morphine hydrochloride, intraperitoneal injection (ip) of naloxone hydrochloride (4 mg·kg -1 ), observe and score all withdrawal symptoms w...

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PUM

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Abstract

The present invention discloses an application of flavone compound or its derivative in preparation of medicine for curing psychic disease and nervous disease. Said invention also discloses a medicine composition containing flavone compound or its derivative. Said invention also provides an excitant of dopamine transport protein firstly, and the tests show that the dopamine transport protein can be excited.

Description

technical field [0001] The present invention relates to the field of biomedicine, more specifically, the present invention relates to a class of dopamine transporter agonists and applications thereof. Background technique [0002] Mental and neurological disorders seriously affect the health of millions of people around the world. Some common mental and / or neurological diseases such as anxiety disorders, mood disorders, schizophrenia, personality disorders, psychosexual disorders, Drug abuse and dependence affect people of all ages, and the duration of these disorders can range from weeks to decades. [0003] With the development of neurobiology and molecular biology, people gradually understand the role of some biochemical factors that cause mental and neurological diseases. Studies have found that important neurotransmitters such as dopamine (DA), norepinephrine (norepinephrine), and serotonin (serotonin) play key physiological roles in mental and neurological diseases. ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P25/18A61P25/30A61P25/00
CPCA61K31/352A61K31/7048A61P15/12A61P25/00A61P25/14A61P25/16A61P25/18A61P25/20A61P25/22A61P25/28A61P25/30
Inventor 郭礼和赵刚许明敏王雪松臧绍云
Owner 上海国联干细胞技术有限公司
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