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Method for preparing lonicera macranthoides hypo-saponin B and application of the same in curing liver cancer, breast carcinoma and cervical cancer

A technique for treating liver cancer with gray felt honeysuckle, applied in the field of medicine, can solve the problems of difficulty in popularization and application and high prices of finished medicines, and achieve the effects of increasing immunity and prolonging survival period.

Inactive Publication Date: 2007-12-12
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the natural anti-cancer active ingredients discovered so far are all trace components in plants, so the price of finished medicines is high, and it is difficult to popularize and apply them. How to develop high-efficiency and cheap anti-tumor drugs is still an extremely urgent and important global issue.

Method used

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  • Method for preparing lonicera macranthoides hypo-saponin B and application of the same in curing liver cancer, breast carcinoma and cervical cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 1 kg of dried flower buds of Lonicera pilosula, extract 3 times with 40% ethanol under reflux, combine the extracts, decolorize with 0.1% medicinal active carbon, filter, and after recovering ethanol, put the medicinal solution directly on the macroporous resin column, first Wash the column with water and 20% ethanol, then elute with 30%, 60%, and 90% ethanol in sequence, recover ethanol under reduced pressure, add 1% NaOH solution, degrade at 100°C for 2 hours, add 1% hydrochloric acid to adjust the pH value to 7. After letting it cool, filter the insoluble matter, dissolve it with 60% ethanol, add 5% medicinal activated carbon for decolorization, filter, recover the solvent under reduced pressure, place it, crystallize, filter, and dry to obtain Lonicera tomentosaponin Second.

Embodiment 2

[0018] 1 kg of dried flower buds of Lonicerae pilosula, reflux extraction with 80% ethanol for 3 times, combine the extracts, decolorize with 3% medicinal activated carbon, filter, recover the ethanol, put the liquid medicine directly on the macroporous resin column, and first use water and 20% ethanol to wash the column, then eluted with 30%, 60%, and 90% ethanol in turn, recovered ethanol under reduced pressure, added 5% NaOH solution, degraded at 80°C for 3 hours, added 5% sulfuric acid to adjust the pH value to 7 , let it cool, filter the insoluble matter, dissolve it with 80% methanol, add 3% medicinal activated carbon for decolorization, filter, recover the solvent under reduced pressure, place it, crystallize, filter, and dry to obtain Lonicera tomentosa saponin B .

Embodiment 3

[0020] 1 kg of dried honeysuckle flowers, reflux extraction with 90% ethanol for 3 times, combine the extracts, decolorize with 5% medicinal active carbon, filter, recover the ethanol, put the liquid medicine directly on the polyamide column, first use water and Wash the column with 20% ethanol, then elute with 30%, 60%, and 90% ethanol in turn, recover ethanol under reduced pressure, add 10% NaOH solution, degrade at 50°C for 4 hours, add 10% hydrochloric acid to adjust the pH value to 7, After letting cool, filter the insoluble matter, dissolve it with 95% ethanol, add 0.1% medicinal activated carbon for decolorization, filter, recover the solvent under reduced pressure, place, crystallize, filter, and dry to obtain Lonicera tomentosaponin B.

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Abstract

The invention relates to a method for preparing Lonicera macranthoides saponin and its application in treating liver cancer, breast cancer and carvical carcinoma. Said method comprises following steps: extracting Lonicera macranthoides and bud with ethanol, decoloring with active carbon, grading in macroreticular resinous column or polyamide column, washing with ethanol, basic hydrolyzing, decoloring with active carbon for the second time, crystallizing and getting said product. The invention is characterized by high productivity and purity of effective component, which is about 98%, and is especially for large- scale production. The effective dosage of Lonicera macranthoides saponin is toxic to external cancer cell, and inhibitive to mouse liver cancer H22, mouse S180 cancer and Lewis lung cancer.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method of Lonicera cinerea Lonicera saponin B and its application in the field of medicine, especially the application in the preparation of drugs for the prevention and treatment of liver cancer, breast cancer and cervical cancer. Background technique [0002] Tumors are a large group of frequently-occurring and common diseases that seriously threaten people's lives and health. Every year, 7 million people in the world develop tumors, 5 million people are killed by tumors, and there are more than 10 million cancer patients. In my country, tumors have become more and more serious health problems. In the early days of liberation, tumors only accounted for the ninth or tenth cause of death in the population. By the 1970s, it had risen to the third place. It is currently estimated that the number of cancer patients in the country is about 1.6 million, the number of peo...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61K9/00A61K36/355A61P35/00
Inventor 冯煦赵兴增王鸣贾晓东董云发陈雨
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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