On-line cell micro observation instrument for biochemical reactor

Abstract

This invention involves an online cell microscopic observation instrument for biochemical reactor, including the main instrument of the observation, the objective lens, the observation window incident, sampling devices, external light source system. Its characteristics are: reflectance prism is installed between external light source and lens system. There is a reflecting mirror behind the objective lens and in the front of entrance window. Reflecting prism is placed in the front of annular aperture films. the upper side of the reflecting prism has a home or area array CCD image sensor. Sampling device includes sampling block, elastic components, a mobile device. The elastic element is installed between sampling block and the drive shaft of mobile devices. the gab between the front face of sampling block and observation entrance window develops the sampling pool. The external light source system of this invention can easily replace wavelength through different filter replacement; dark field of view at the same time lighting can improve resolution of observation instrument for the cells into the contrast of the image and resolution; and it is simple in structure, easy-to-use, manufacturing costs low.

Description

technical field [0001] The invention relates to an on-line cell microscopic observer, in particular to an on-line cell micromorphological observer applied to a biochemical reaction tank. Background technique [0002] When living cell culture is used for product production in the biological reaction process, cell growth and morphological changes are extremely important monitoring parameters. For a long time, the method of sampling from the tank was obtained by offline microscope observation. In recent years, with the advancement of electronic technology, images can be converted into digital signals for computer processing, thereby obtaining quantitative information on growth and morphological changes. However, the above method is only off-line interval sampling, lack of dynamic observation, especially in the large-scale cultivation process of animal cells, anti-bacterial contamination is the primary problem of process operation, so frequent sampling is impossible, resulting i...

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Problems solved by technology

This scheme adopts the bright field illumination method, which has poor contrast and resolution of the image formed by the transparent cells; and because the high-speed camera must be used when the stirring speed of the reaction tank is fast, and there is no sampling device to limit the depth of field, so it is different from that in the figure. The solution shown in 1 cannot eliminate the effect on the depth of field; at the same time, because the light source uses a complex SLD (Super Light Diode), the light source drive controller is relatively complicated, so it is costly and difficult to replace the light source and switch the excitation wavelength

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