HCV composite multi-epitope transgene plant oral vaccine

A plant vaccine and multi-epitope technology, which is applied in the field of transgenic edible vaccines to prevent human hepatitis C virus infection, can solve the problems of reducing immunity and not using complex multi-epitope genes, and achieves multivalent storage and easy storage. , strong immunogenic effect

Inactive Publication Date: 2007-11-14
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although their expression system is an edible plant, they did not use a compound polyepitope gene, which reduces the immunity of the product
[0010] At present, there is no expression of HCV compound multi-epitope antigen gene in edible plants

Method used

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Experimental program
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Embodiment Construction

[0050] The content of the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the content of the present invention is not limited thereto.

[0051] 1. Gene construction: according to the above-mentioned sequence design, its specific sequence is as follows

[0052] 1) Synthesize a sequence CH-1 according to the following sequence, which includes: HCV highly conserved sequence core protein (1~16aa, 132~141aa),

[0053] E1(317~326aa), NS3(1445~1453aa) and NS5(2781~2788aa) and hepatitis B pre-S T cell activation epitope. Insert this sequence into the cloning vector pUC18, and name this vector pCH1.

[0054] M S P Q A Q G M L K T L P

[0055] ATG TCT CCA CAA GCT CAG GGA ATG TTG AAG ACT CTT CCA

[0056] A D P P P A S T Y R Q S G

[0057] GCA GAT CCT CCA CCT GCT TCT ACA TAT AGA CAG TCA GGA

[0058] R Q g p M T S T N P K P

[0059] AGA CAA GGT CCT ATG ACA TCT ACA AAT CCA AAG CCA

[0060] Q K T K R ...

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Abstract

This invention is one kind of hepatitis C virus (HCV) compound multi-epi-position transgene edibility plant vaccine. States the HCV vaccine is the HCV compound multi-epi-position antigen gene, in the plant expresses in the host-based system to express obtains, and states the plant expresses the host is edibility. This invention provided one kind highly effective moderately-priced in view of the same type, different HCV separation not to be able to provide the overlapping protective function the effective HCV vaccine.

Description

1. Technical field [0001] The invention belongs to the field of molecular biology and relates to an edible transgenic vaccine prepared by using plants, in particular to a transgenic edible vaccine for preventing human hepatitis C virus infection; the amino acid sequence of the recombinant protein; encoding the recombinant protein The nucleotide sequence of the vaccine (hereinafter sometimes referred to as "gene"); the expression vector containing the nucleotide sequence; the host cell containing the expression vector. 2. Background technology [0002] Hepatitis C is an infectious disease that seriously affects human health. It can easily transform into liver cirrhosis and hepatocellular carcinoma. At present, there is no ideal prevention and treatment method. It is one of the important sources of disease that endangers human health. There are about 170 million HCV infected people in the world, and it is estimated that my country accounts for more than 40 million. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12N15/51C12N15/82A61K48/00A61P1/16A61P31/14
Inventor 刘均洪李凤梅石琰景张媛媛苏忠亮李俊峰吕辉
Owner QINGDAO UNIV OF SCI & TECH
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