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Artificial rapid reproduction method for rutaceae zanthoxylum plant zanthoxylum dissitum Hemsl

A technology of the genus Zanthoxylum and Rutaceae, applied in the field of medicine, can solve the problems of slow growth, lack of natural resources, low natural reproduction rate of single-sided needles, etc.

Active Publication Date: 2007-10-24
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is mainly distributed in Southwest my country, Guangdong, Guangxi, Hunan, Hubei, Shaanxi and other places. In Hunan Province, it is mainly distributed in western Hunan. Slow, resulting in the increasingly scarce natural resources, in short supply, and gradually threatening the normal production of related pharmaceutical factories

Method used

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  • Artificial rapid reproduction method for rutaceae zanthoxylum plant zanthoxylum dissitum Hemsl
  • Artificial rapid reproduction method for rutaceae zanthoxylum plant zanthoxylum dissitum Hemsl

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 primary culture (establishment of aseptic system)

[0014] 1. Primary culture (establishment of sterile system)

[0015] 1.1 Material selection: Cut the seed shell with a scalpel, take out the embryo from the embryo sac, then disinfect with 70% alcohol, then disinfect the surface with 0.1% mercury chloride, rinse with sterile water for 3 to 4 times, and then use Blot dry with sterile filter paper or gauze.

[0016] 1.2 Preparation of medium: Prepare MS medium according to the culture formula, adjust the pH value to 5.8-6.0, pack it in a 50mL triangular flask, place it in an LDZX-40II automatic electric heating pressure steam sterilizer, and set the temperature at 1.06kg / cm 2 Sterilize at 121°C for 25 minutes under pressure. (conventional preparation)

[0017] 1.3 Effects of different hormones and concentrations on in vitro embryo culture

[0018] In the experiment, isolated single-sided needle embryos were used as explants, cultured with MS solid medium...

Embodiment 2

[0031] Embodiment 2 Subculture (proliferation and cultivation of tissue culture seedlings)

[0032] 1.1 Preparation of medium (conventional preparation)

[0033] Basic medium MS, WPM, B 5 , White four, additional components BA, ZT, KT, CPPU, NAA, IAA, IBA additives, etc., pH value 5.8 ~ 6.2, agar 7.5g / L, sucrose concentration 30g / L, additional components according to cytokinin and Different ratios of auxin were added to the basic medium. Divide the culture medium into 300mL wide-mouth bottles, wrap each bottle with 30mL parafilm, and store at 121°C at 1.1kg / cm 2 Sterilize in a high pressure steam sterilizer for 21 minutes.

[0034] 1.2 Experimental materials

[0035] The aseptic seedlings cultured in vitro by single-sided needles are used as explants, and the single-bud aseptic seedlings with terminal buds and no axillary buds are cut into a length of 1-1.5 cm, inoculated into the medium for subculture, Proliferation culture for 30d.

[0036] 1.3 Effects of different bas...

Embodiment 3

[0068] Embodiment 3 strong seedlings and rooting culture

[0069] Strong seedling cultivation must be carried out before rooting cultivation. Strong seedling medium: MS+ZT 2.0mg / L+NAA 0.2mg / L.

[0070] 1.1 The effect of basic medium on the rooting of tissue culture seedlings

[0071] The basic medium was 1 / 2MS, 1 / 2WPM, 1 / 2B5, 1 / 2White, and IBA1.0mg / L was added respectively, and the tissue culture seedlings with strong growth were selected and inoculated in the four kinds of medium, and there were 5 treatments in total, each Treatment inoculated 25 cluster buds, and the experiment was repeated 3 times. After 30 days, the rooting rate was counted, and the average value was taken for single factor analysis.

[0072]After a single cluster bud was inoculated on 4 different basal media with 1.0mg / L of Annex IBA for 15-20 days, color-preserving callus could be seen at the incision of the cluster bud on each medium, but adventitious roots had not yet differentiated. With the prolo...

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Abstract

The invention relates to a manual method for fast breeding Zanthoxylum nitidum var. fastuosum How ex Huang. It employs tissue culturing technology, disinfects the isolated embryo and produces large amount of tissue seedling through initial culture, subculture, strong seedling and rooting culture, replants after hardening seedling and realizes fast breeding of Zanthoxylum nitidum var. fastuosum. The invention is characterized in that it designs a series of culture medium aiming at tissue culture of Zanthoxylum nitidum var. fastuosum, which greatly increases germination rate of isolated embryo and breeding efficiency and rooting rate, and thus meets increasing demand for Zanthoxylum nitidum var. fastuosum from Chinese patent drug. The method is simple, culture medium formation is specific, and the operation is practicable.

Description

technical field [0001] The invention relates to the field of medicine, in particular to an artificial rapid propagation method of a plant of the genus Zanthoxylum rutaceae. Background technique [0002] Zanthoxylum Dissitum Hemsl, also known as shell pepper, mountain loquat, big-leaf pepper, clamshell pepper, etc., belongs to the Rutaceae Zanthoxylum evergreen climbing or vine woody shrub. Roots, stems and leaves can be used as medicine. Clinically, it has the effects of expelling wind and activating collaterals, dissipating blood stasis, relieving pain, detoxifying and reducing swelling. It is mainly used to treat toothache, low back pain, women's menorrhagia, postpartum irregular menstruation and other diseases; folks are used to treat waist and leg pain , joint pain, bruises embolism. It is mainly distributed in Southwest my country, Guangdong, Guangxi, Hunan, Hubei, Shaanxi and other places. In Hunan Province, it is mainly distributed in western Hunan. Slowly, the natur...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G31/00
Inventor 王平左之文马英姿颜利玲
Owner ZHUZHOU QIANJIN PHARMA
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