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Method for preparing MDG-1 polysaccharide of lilyturf root

A technology of Ophiopogon japonicus polysaccharide and MDG-1, applied in the field of preparation of Ophiopogon japonicus polysaccharide MDG-1, can solve the problems of high cost, time-consuming, inability to prepare a large amount of Ophiopogon japonicus polysaccharide, etc. Effect

Inactive Publication Date: 2007-10-10
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preparation method is time-consuming and costly, and cannot be adapted to industrial production, nor can it be used for large-scale preparation of Ophiopogon japonicus polysaccharide MDG-1

Method used

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  • Method for preparing MDG-1 polysaccharide of lilyturf root
  • Method for preparing MDG-1 polysaccharide of lilyturf root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 (preparation of Ophiopogon japonicus polysaccharide MDG-1)

[0022] 1. Extraction of Ophiopogon japonicus polysaccharide

[0023] Weigh 20kg of dried Ophiopogon japonicus roots, extract 10 times the amount with water for 3 times, each time for 30 minutes, and extract the dregs with water twice. Combine the water extracts and concentrate them under reduced pressure to 1g / ml, add 95% ethanol until the alcohol content reaches 70%, let stand overnight, pour off the supernatant, repeat the precipitation with alcohol once, and freeze-dry to obtain light yellow Ophiopogon japonicus crude polysaccharide powder.

[0024] 2. Ultrafiltration separation of Ophiopogon japonicus polysaccharide

[0025] Dilute the crude polysaccharide of Ophiopogon japonicus 15 times, use a flat ultrafiltration membrane with a molecular weight cut-off of 10,000 and the material is cellulose acetate for ultrafiltration, the ultrafiltration pressure is 0.3MPa, and the ultrafiltrate is con...

Embodiment 2

[0031] Embodiment 2 (preparation of Ophiopogon japonicus polysaccharide MDG-1)

[0032] 1. Extraction of Ophiopogon japonicus polysaccharide

[0033] Weigh 20kg of dried Ophiopogon japonicus roots, extract 8 times the amount with water for 3 times, each time for 30 minutes, and extract the dregs with water for 3 times. Combine the water extracts and concentrate them under reduced pressure to 0.5g / ml, add 75% ethanol until the alcohol content reaches 60%, let it stand overnight, pour off the supernatant, repeat the precipitation with alcohol once, and freeze-dry to obtain light Yellow Ophiopogon japonicus polysaccharide powder.

[0034] 2. Ultrafiltration separation of Ophiopogon japonicus polysaccharide

[0035] Dilute the Ophiopogon japonicus crude polysaccharide 15 times, use a hollow fiber ultrafiltration membrane ultrafiltration with a molecular weight cut-off of 10,000 and the material is polyamide, and the pressure is 0.1MPa. The ultrafiltrate is concentrated at 70°C a...

Embodiment 3

[0041] Embodiment 3 (preparation of Ophiopogon japonicus polysaccharide MDG-1)

[0042] 1. Extraction of Ophiopogon japonicus polysaccharide

[0043]Weigh 20kg of dried Ophiopogon japonicus tuber, extract 12 times the amount with water for 3 times, each time for 30 minutes, and extract the dregs with water for 2 times. Combine the water extracts and concentrate them under reduced pressure to 0.75g / ml, add 95% ethanol until the alcohol content reaches 80%, let stand overnight, pour off the supernatant, repeat the precipitation with alcohol once, and freeze-dry to obtain light Yellow Ophiopogon japonicus polysaccharide powder.

[0044] 2. Ultrafiltration separation of Ophiopogon japonicus polysaccharide

[0045] Dilute the crude polysaccharide of Ophiopogon japonicus 15 times, use a tubular membrane ultrafiltration with a molecular weight cut-off of 10,000 and the material is sulfonated polysulfone, the pressure is 0.2MPa, and after the ultrafiltrate is concentrated at 70°C, f...

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Abstract

This invention discloses a method for preparing Ophiopogon japonicus polysaccharide MDG-1 with anti-myocardial ischemia function. The method comprises: extracting Ophiopogon japonicus roots with water, precipitating with alcohol to obtain crude polysaccharide, separating by ultrafine filtration, eluting the concentrate with water in a macroporous weak-base anion exchange resin, collecting eluates 4-8 volume times of the column, incorporating the eluates, and drying to obtain Ophiopogon japonicus polysaccharide MDG-1 with purity greater than or equal to 90%. The method has such advantages as short time and is suitable for mass production.

Description

technical field [0001] The invention relates to a preparation method of Ophiopogon japonicus polysaccharide MDG-1 with anti-myocardial ischemia effect. Background technique [0002] Yin-nourishing traditional Chinese medicines have the effects of promoting body fluid and moistening dryness, nourishing yin and nourishing liquid, etc. They are mainly used to treat yin deficiency syndromes. They are generally less toxic and have mild effects. disease is of great importance. With people's understanding of the pharmacological effects of polysaccharides, more and more attention has been paid to the research on polysaccharides. Traditional Chinese medicines with the effect of nourishing yin often contain a large amount of polysaccharides. Blood sugar, hypolipidemic effect, anti-oxidation, anti-aging, hepatoprotective effect, anti-viral effect, anti-fatigue, hypoxia resistance, anti-fibrosis, anti-ulcer effect, etc. [0003] Ophiopogon japonicus polysaccharides with a molecular we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P9/00
Inventor 冯怡徐德生林晓
Owner SHANGHAI UNIV OF T C M
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