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Preparation method of oyster active peptides

A technology of active peptides and oysters, applied in the fields of peptide sources, animal/human peptides, anti-tumor drugs, etc.

Inactive Publication Date: 2007-09-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, developed countries such as Europe, Japan, and the United States have increasingly conducted research on biologically active substances in oysters, but there have been no reports of actually finding substances with significant biological activity from oysters and developing them as medicines

Method used

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Experimental program
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Effect test

Embodiment 1

[0010] Shell the oysters, take the oyster meat, wash with 4°C water, homogenate, add distilled water at a low temperature (4°C) for extraction for 2 hours according to the weight ratio of 1:1, flocculate, centrifuge to remove slag, take the supernatant, and After the supernatant was lyophilized, it was separated with a Sephacryl s-100 HR column (0.03mol / lNH 4 HCO 3 elution buffer), after collecting each peak respectively, take the peak with biological activity and then use anion agarose gel DEAE-Sepharose-FF ion exchange column chromatography (PH8.5 Tris-HCl buffer solution, 0~0.3mol / lNaCl Gradient elution), the active peaks were collected, freeze-dried, and desalted with Sephadex G15 gel column to obtain pure natural active oyster polypeptide.

Embodiment 2

[0012] Shell the oysters, wash, homogenize, add distilled water according to the weight ratio of 1:1, and add trypsin according to the weight ratio of 2% of the substrate, enzymatically hydrolyze at 45°C for 4 hours, inactivate the enzyme, centrifuge to remove the slag, and freeze-dry to obtain the enzyme Solve the crude extract, carry out gel column chromatography with Sephadex G25 for the crude extract, collect each peak, then use anion agarose gel DEAE-Sepharose-FF ion exchange column chromatography to separate and purify (PH8. 5Tris-HCl buffer solution, 0~0.3mol / l NaCl gradient elution), collect the active peaks, after lyophilization, use Sephadex G15 gel column to desalt, and obtain the pure product of oyster natural active polypeptide. The active peptide is a naturally extracted substance, belonging to an oligopeptide with a small molecular weight, and its biological activity is effectively protected during the extraction process.

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Abstract

The invention relates to a method for hydrolyzing oyster protein by enzyme method and extracting oyster active peptide by column chromatography including: rinding the oyster, washing, homogenate, adding distilled water to extract the oyster liquid at a low temperature; flocculating, centrifugal deslagging, taking the supernatant fluid, freeze-dry the supernatant fluid, separateing with the Sephacryl s-100 HR column or enzymolysis, inactivating, centrifugal deslagging, freeze-dry the oyster liquid to get coarse extract, gel column chromatography of the coarse extract; collecting the respective peak, purifying with ion-exchange column chromatography, collecting the respective peak having activity, desalting with the gel column after freeze out and getting the oyster natural purified activity peptide. The oyster natural activity peptide has a high purity and has an anti-tumor activity. The active peptide is natural-extracted and is protected in the extraction process and may be a medicine to use.

Description

Technical field: [0001] The invention relates to a preparation method of oyster active peptide, specifically a method for hydrolyzing oyster protein by enzymatic method and separating and purifying oyster active peptide by ultrafiltration and column chromatography, belonging to the technical field of marine natural products and medicine. Background technique: [0002] Oyster (Oyster) is a kind of marine shellfish. Oysters belong to Molluscs, Phallobranches, Heterocylinda, Oyster Superfamily, and Oysteridae in classification. It is a bivalve seafood that is used for both food and medicine. It is cultivated in the north and south coasts of my country, especially in Guangdong Province, which has developed rapidly in recent years. The oyster meat is milky white, tender, and rich in nutrients. In addition to rich protein, vitamins, sugar and other nutrients, it also contains more than ten essential nutrients. Amino acids, mineral nutrients, etc. Current studies have also proved ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C12P21/00C07K14/435A61P35/00
Inventor 韩玉谦冯晓梅赵志强蒋新管华诗
Owner OCEAN UNIV OF CHINA
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