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Use and method for predicting serotonin re-uptake inhibitor medicine effect by polymorphic site

A technology of serotonin reuptake and polymorphism site, applied in the field of medicine, can solve problems such as poor curative effect, inability to improve drug curative effect, toxic and side effects, and delayed treatment timing.

Inactive Publication Date: 2007-09-05
BEIJING HUAANFO BIOMEDICAL RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that patients with depression who are homozygous for the 7th intron 218A allele of TPH are less effective in treatment with fluvoxamine [MolPsychiatry.2001 Sep; 6(5): 586-92]
Another study showed that patients with depression who were homozygous and heterozygous for the 218A allele in intron 7 of TPH were less effective in treatment with paroxetine [Eur Neuropsychopharmacol.2001 Oct; 11(5): 375-80]
[0015] Due to individual differences in drug reactions, doctors cannot choose drugs and drug compatibility according to the individual's needs when choosing drugs, and cannot improve the efficacy of drugs and reduce the occurrence of toxic and side effects, which may delay the timing of treatment and cause economic losses to patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Determination of the C1019G (dsSNP ID: rs6295) polymorphic site genotype of the HTR1A gene and prediction of the effect of serotonin reuptake inhibitor drugs

[0060] (1) Determination of the genotype of the C1019G (rs6295) polymorphism site of the HTR1A gene:

[0061] (1) Extract the genomic DNA of the host cell:

[0062] (a) Add 30ml of erythrocyte lysate to the whole blood, shake slowly, and let stand at room temperature for 10 minutes. During this period, shake several times to completely lyse the erythrocytes;

[0063] (b) Centrifuge at 4°C and 2000 rpm for 10 minutes, remove the supernatant, break up the precipitated white blood cells on a rotary shaker, add 40ul of protease and 50ul of RNase, shake well, add 15ml of white blood cell lysate, and mix Take it out after evenly bathing in 37°C water bath for 20 minutes, and put it in cold water;

[0064] (c) Add 4ml of cold protein precipitation solution, mix well and place in -20°C refrigerator for 5 min...

Embodiment 2

[0156] Example 2: Determination of the C / T (rs878567) polymorphism site genotype of the HTR1A gene and prediction of the effect of serotonin reuptake inhibitor drugs

[0157] (1) Determination of the genotype of the C / T (rs878567) polymorphism site of the HTR1A gene:

[0158] (1) According to the conventional operation process, adopt the method similar to Example 1 to extract the genomic DNA of the host cell

[0159] (2) Use the Taqman method to detect the genotype of the C / T (rs878567) polymorphism site of the HTR1A gene

[0160] (a) Amplify the HTR1A functional gene polymorphic site and its flanking sequences with a PCR instrument, containing 10ng of genomic DNA in a 5ul PCR reaction system, and 2.5ul of Taqman 2X Universal PCR Master Mix No AmpEraseUNG (composition includes: AmpliTaq Gold DNA Polymerase, dNTPs with Dutp, PassiveReference, optimized buffer), and 0.72uM forward primer, 0.72uM reverse primer and two allele-specific probes with fluorescent reporter groups each...

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PUM

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Abstract

A method for determining HTR1A gene polymorphism site gene and predicting 5-hydroxytrytamine re-ingestion inhibitor medicinal effect and its use are disclosed. It can predict therapeutical effect and acting time by polymorphism site oligonucleotide inspecting biological sample. It's safe, cheap and non-toxic and can be used for thymoleptic and medicine in treatment of sexual disorders.

Description

technical field [0001] The present invention relates to the use and method of polymorphic site genotype prediction of the effect of serotonin reuptake inhibitor drugs, especially comprising at least C1019G (rs6295), C / T (rs878567), A / The utility and method for predicting the effect of serotonin reuptake inhibitor drugs by polymorphic site in G(rs6449693) belong to the field of medicine. Background technique [0002] Depression is one of the most common mental disorders. According to surveys in the United States and other developed countries, the lifetime prevalence of depression is 10-25% in women and 5-12% in men. The ratio of men to women is about 1: 2. The prevalence of depression in my country is at least not less than 1% ["Psychiatry". Fourth Edition. People's Health Publishing House. 1 1994], estimated to be around 3% to 5%. According to statistics from the Ministry of Health [Lancet, 2004, 364: 18-24], about 280,000 people die by suicide in my country every year. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A61K45/00A61P25/24A61P15/10G01N33/53
Inventor 刘平王玉洪秀梅张善春王滨燕王燕徐希平
Owner BEIJING HUAANFO BIOMEDICAL RES CENT
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