Rapid identification method for genetic purity of cabbage seed

Abstract

This invention discloses one cabbage seed heritage purification rapid test method in biological technique field, which comprises the following steps: extracting cabbage gene set DNA and using filtered property lead production of NAUISR1034 and NAUSSR1011 for PCR extending ; processing extended DNA section for agarose gel and non-variable polyacrylamide gel electrophoresis for 0.8 to 1 and 2 to 2.2 to catch electrophoresis spectrum; through comparing and analyzing the DNA section difference to process new summer 50 mixture heritage purification identification.

Description

Technical field [0001] The invention relates to a method for quickly identifying the genetic purity of cabbage seeds, which uses ISSR and SSR molecular marker technology to detect the genetic purity of cabbage variety Xinxia 50 seeds, and belongs to the field of biotechnology. Background technique [0002] In the process of vegetable hybrid seed production, the selfed seeds of the female parent line are often mixed with F 1 Medium, seriously affecting the quality and purity of seeds. At present, hybrid seeds of cabbage are mainly produced by self-incompatible lines or male sterile lines. However, due to the defects of self-incompatibility and the problem of micronization of male sterility, the female parent’s self-breeding often leads to false hybrids ( Crockett, PA, Bhalla, PL, Lee, CK, Singh, MB. RAPD analysis of seed purity in a commerical hybrid cabbage (Brassica oleracea var. capitata) cultivar. Genome 2000, 43, 317-321.), which will give breeding Workers, producers, sellers...

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Problems solved by technology

[0003] At present, the purity detection of cabbage varieties mainly includes field morphological identification and isoenzyme identification. However, field morphological identification requires sowing and raising seedlings, transplanting them into the field, and then observing the morphological characteristics, which takes a long time and a lot of work. impact, high cost

And sometimes, because the morphological differences between cabbage pseudo-hybrids and true hybrids are not obvious, it is not easy to identify at the seedling stage and adult plant stage, especially when the genetic basis between the parents is very close, it is even more difficult to identify

Isozyme analysis is easily affected by the environment and the parts of the materials taken, and for some hybrid varieties with small genetic differences between the parents, it is sometimes difficult to perform purity detection and variety identification of isozymes, and the accuracy is not high (Liu Liwang, Hou Xilin, Gong Yiqin, Zhang Yuming, Wang Kairong, Zheng Junfei. The role of molecular marker technology in the identification and purity detection of vegetable crops. Molecular Plant Breeding, 2004, 2(4): 563~568.)

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