Small-amount free cell or tiny sampler collector

Abstract

The invention discloses a small amount of free cells or small samples collector belonging to the TEM sample production technology area. The collector includes a conical collection cavity, and the cavity of the conical collection cavity is divided into upper and lower parts; a shell; the cavity of the conical collection cavity is placed and fixed within the shell. The upper cavity of the collection cavity makes up of the rigid material, for holding the samples suspension; the lower cavity is made of the gel, used for the sample enrichment. Transparent hard shell is used for inclusive collection cavity, and bearing the centrifugal force of the collection cavity and the sample suspension. Using the collector, it can make the centrifugal sedimentation samples in the gel cavity directly buried, effective solving the problem that a small amount of samples are difficult to collect and pick up, lowering the requirements of the TEM sample preparation to the initial sample volume, and only needing a 1 X 103 yeast, it can produce cell block visible to the naked eye in the gel buried block, and convenient for the repair, cutting, and other operations, and it provides a new sample collection method for electron sample preparation.

Description

technical field [0001] The invention belongs to the technical category of transmission electron microscope sample preparation, and particularly relates to a collector for collecting a small amount of free cells or tiny samples (such as subcellular structures) in a suspension. Background technique [0002] In biological research, a limited number of samples are often obtained, which increases the value of the sample, even extremely precious, and causes considerable losses if it is wasted. However, for free cells or tiny samples (such as subcellular structures, etc.), the preparation of TEM samples usually requires a large number of samples. For example, the initial number of cells required to make a microbial electron microscope sample is usually 1×10 7 Above, the minimum can not be less than 1×10 5 . This is because in the process of electron microscope sample preparation, there will usually be a loss in the number of samples; secondly, if the number of samples is too sma...

AI Technical Summary

Problems solved by technology

This is because in the process of electron microscope sample preparation, there will usually be a loss in the number of samples; secondly, if the number of samples is too small, the sample clumps formed after centrifugation will be very small, which is not conducive to sample picking; thirdly, even if sometimes The sample can be picked up, but after multiple treatments such as fixation, rinsing, dehydration, and soaking, the sample is difficult to identify in the resin-embedded block, and the trimming operation cannot be performed

If you rashly make TEM samples of a small number of cells or tiny samples by conventional methods, the test will often fail due to the difficulty of collecting the sample, picking it up after collecting it, or slicing it after picking it up, resulting in irreversible consequences

[0003] The limitations of the existing electron microscope sample collection methods limit the collection, sample preparation and electron microscope observation of a small amount of free cells or tiny samples, which restricts the development of related scientific research work

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