Process for preparing non-alcohol fatty liver mouse model

Abstract

The invention relates to a method for preparing non-alcohol fatty hepatic disease mouse model, which comprises that randomly distributing the SPF male C57BL / 6 mouse at 18-25g into model group and normal contrast group, while each group has 36 mouse; two groups can be divided into six groups via different feeding times, as 1 week, 2, weeks, 3 weeks, 4 weeks, 5 weeks, and 8 weeks, while each time point has six mouse; all mouse are fed via normal contrast forage, then to be fed via bursine-acimetion lack forage, while the contrast group is fed via normal contrast forage; weighting them in each week, killing at first, second, third, fourth, fifth, and eighth week, to evaluate the model condition. The inventive model has high stability, integral disease spectrum, and high speed.

Description

technical field [0001] The invention relates to a method for preparing a mouse model of nonalcoholic fatty liver disease. Specifically relates to a preparation method of an animal model used for research on the pathogenesis and treatment of non-alcoholic fatty liver disease. Background technique [0002] In recent years, with the improvement of living standards, the prevalence of non-alcoholic fatty liver disease has increased year by year. It has reached about 15% in my country, and it is on the rise; in western developed countries, it is as high as 20%-30%, becoming the primary cause of chronic liver disease. Non-alcoholic fatty liver disease itself will progress to liver cirrhosis and even liver cancer, and it will also accelerate the progress of chronic hepatitis and other liver diseases to liver cirrhosis and liver cancer; non-alcoholic fatty liver disease can also promote diabetes, coronary The development of heart disease leads to a high incidence of metabolic syndr...

AI Technical Summary

Problems solved by technology

[0004] The existing animal models of non-alcoholic fatty liver disease, such as ob / ob, db / db mice and Obese Zucker rats and other genetically derived spontaneous animal models, mostly need to be imported from abroad, are expensive, and require high feeding conditions , and these animal models cannot spontaneously reproduce non-alcoholic steatohepatitis due to the lack of leptin gene; while the non-alcoholic fatty liver disease animal model reproduced by feeding SD rats with a high-fat diet can reproduce non-alcoholic fatty liver disease Steatohepatitis, but the molding speed is slow, generally takes about three months, and the stability of the model is not good

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