Method for evaluating lung infection medicine
An evaluation method and technology for pulmonary infection, applied in antibacterial drugs, medical raw materials derived from bacteria, etc., can solve the problems of difficulty in identifying infectious bacteria or colonized bacteria, lack of animal models, etc., and achieve significant curative effect, strong feasibility, and operation. simple effect
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[0031] 1. Materials
[0032] 1.1 Source of strain: The imipenem-resistant Acinetobacter baumannii used in the experiment was isolated from sputum samples from the First Affiliated Hospital of Zhejiang University School of Medicine, and identified by WITEK-II and OXA-51-like gene sequencing.
[0033] 1.2. Culture medium: blood plate, produced by Mérieux, France; M-H agar, produced by Columbia.
[0034] 1.3 Experimental drug: methotrexate for injection: produced by Jiangsu Hengrui Medicine Co., Ltd., batch number: 07072812;
[0035] 1.4. Experimental animals: SPF grade BALB / C mice, aged 4-5 weeks, weighing 13-14g, male, purchased from Zhejiang Experimental Animal Center, certificate number: SCXK (Zhejiang) 2003-0001.
[0036]1.5. Instruments and equipment: ultrasonic nebulizer, Jiangsu Yuyue Medical Instrument Co., Ltd., VITEK-AMS automatic microbial analyzer. SW-II-A / B 3 Biological safety cabinet: Shanghai Shangjing Purification Equipment Co., Ltd. Atomized plastic barrels ...
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